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中国农学通报 ›› 2006, Vol. 22 ›› Issue (8): 203-203.

所属专题: 生物技术 小麦

• 目次 • 上一篇    下一篇

黄淮及长江中下游小麦品种HMW-GS等位基因的分布

孙学永,马传喜,司红起,乔玉强,王 维   

  • 出版日期:2006-08-05 发布日期:2006-08-05

Distribution of Alleles of High Molecular Weight Glutenins Subunits of Cultivars in HuangHuai and Yangtse River Area in China

Sun Xueyong, Ma Chuanxi, Si Hongqi, Qiao Yuqang, Wang Wei,   

  • Online:2006-08-05 Published:2006-08-05

摘要: 选取277份来自黄淮、长江中下游地区推广的、育种单位的高代品种(系),以及部分种质资源和国外引进资源,利用SDS-PAGE方法分析了它们的高分子量麦谷蛋白亚基等位基因的组成。结果表明,Glu-A1位点,共检测出1、2*、N三种亚基类型,其中以1亚基的频率最高达到49%,其次为N亚基为44.77%,二者占总品种数的94.22%,2*亚基的频率最小。在Glu-B1位点的等位变异类型最丰富,共检测出8种变异类型,其中以7+9和7+8为最多,分别为38.99%和36.10%,其次为14+15,为12.64%,其它等位亚基所占数目较少。在Glu-D1位点共检测出四种等位类型,其中以2+12亚基类型为最多占44.77%,其次为5+10亚基占33.94%和4+12亚基占19.49%。对各亚基组合的分布,检测出43种组合。其中以N,7+8,2+12在各品种中所占频率为最高,其值为12.64%,其次为N,7+9,2+12,占9.39%;1,7+8,2+12,占7.94%;N,14+15,4+12,占5.42%,其余亚基类型所占比例较低

关键词: 秦川牛, 秦川牛, 生长激素, 胰岛素, 体尺指数, 体重

Abstract: The 277 varieties and lines, which come from HuangHuai and Yangtse River Area in China, were analyzed using SDS-PAGE for its composition of HMW-GS. The results showed that the 3 subunit types which were 1, 2*, N were screened at Glu-A1 locus. Among the three subunits, the frequency of 1 subunit was the highest, its value was 49%; the second was N subunit, its value was 44.77%, the two subunit’s frequency summed to 94.22%. The allele subunits were most abundance at Glu-B1 locus; there were 8 alleles subunit that were screened. Among the subunits, the frequency of 7+8 and 7+9 was the highest value, 38.99% and 36.10% respectively. The next was 14+15, 12.64%, and other subunit’ frequencies were less. 3 subunit types, which were 2+12, 5+10, 4+12, their frequencies were 44.77%, 33.94%, 19.49%, respectively, were identified at Glu-D1 locus. The 43 subunit compositions were identified in the test varieties, the number of varieties included N, 7+8, 2+12 was the highest, its frequency was 12.64%, the second subunit compositions was N, 7+9, 2+12, which accounted for 9.39%; the frequency of subunit compositions included 1, 7+8, 2+12, which accounted for 7.94%; N, 14+15, 4+12 accounted for 5.42%

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