关键词: 土壤质量, 土壤质量

Abstract: This study aimed at high-yield expression of the pcGH gene which was separated from natural large yellow croaker in the yeast Pichia pastoris in order to produce amount of large yellow croaker growth hormone (Pseucdosciaena crocea, Growth Hormone, pcGH) with the feature of biological activity for further use. The pcGH gene was first cloned into the integrative expression vector of methylotrophic yeast P. Pastoris to form the recombinant vector pPIC9K-pcGH, and the linearized recombinant plasmid was then transformed into the chromosome of the HIS4 mutant yeast GS115 by electroporation, and it could finally get high-yield expression induced by methanol. SDS-PAGE confirmed that the expression product was pcGH, and it led to a level as high as of 62.5 mg/L.