欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (13): 60-65.doi: 10.11924/j.issn.1000-6850.casb2021-0544

所属专题： 生物技术； 园艺； 农业生态

• 林学·园艺·园林 • 上一篇    下一篇

欧李高效再生体系的建立

杨文静1(), 王占军2, 甘晓燕1, 何建龙2, 张丽1()   

  1. 1宁夏农林科学院农业生物技术研究中心,银川 750002
    2宁夏农林科学院荒漠化治理研究所,银川 750002
  • 收稿日期:2021-05-24 修回日期:2021-09-17 出版日期:2022-05-05 发布日期:2022-06-08
  • 通讯作者: 张丽
  • 作者简介:杨文静,女,1990年出生,研究实习员,硕士,研究方向为植物分子育种。通信地址：750002 宁夏银川市金凤区黄河东路590号 宁夏农林科学院,E-mail： 429625104@qq.com。
  • 基金资助:
    宁夏回族自治区重点研发计划项目“欧李优新品种引选与产业化关键技术研究示范”(2020BBF02027);自治区农业科技自主创新专项科技创新引导项目“宁夏沙区欧李苗木繁育及配套栽培管理技术研究与示范”(NKYG-18-04);宁夏农林科学院科技平台建设提升项目“欧李种质资源圃资源收集保存与评价”(NKYP-19-07)

The Establishment of Efficient Regeneration System of Cerasus humilis

YANG Wenjing1(), WANG Zhanjun2, GAN Xiaoyan1, HE Jianlong2, ZHANG Li1()   

  1. 1Agricultural Bio-Technology Centre, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002
    2Institute for Desertification Control, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002
  • Received:2021-05-24 Revised:2021-09-17 Online:2022-05-05 Published:2022-06-08
  • Contact: ZHANG Li

RichHTML

5

PDF (PC)

23

摘要：

研究旨在利用植物组织培养技术,筛选出引进资源品种（系）SN6、SN7和自育品系YC24的最适培养基,确定3个欧李品种的再生体系,为欧李高效快速规模化繁殖提供技术性支持。以3个欧李品种（系）为材料,对外植体的消毒方式、分化、增殖和生根培养阶段的最适培养基和激素配比进行研究。结果表明,头孢霉素浓度为100 mg/L时,外植体存活率最高、污染率最低;SN6、SN7的最适分化培养基为MS+ 0.5 mg/L 6-BA+ 1.0 mg/L ZT+ 0.05 mg/L IBA,YC24的最适分化培养基为MS+ 0.5 mg/L 6-BA+ 0.5 mg/L ZT+ 0.05 mg/L IBA;SN6的最适生根培养基为1/2MS+ 0.2 mg/L NAA+ 0.1 mg/L IBA+ 1.0 g/L活性炭,SN7、YC24的最适壮苗生根一步培养基为1/2MS+ 0.05 mg/L NAA+ 0.2 mg/L IBA。本研究建立了欧李嫩枝快速繁殖体系,可为优良种质的后续练苗移栽、扩大培养和推广应用提供参考。

关键词: 欧李, 内生菌, 分化, 生根, 矮壮素, 高效再生

Abstract:

The aim of this study is to use plant tissue culture technology to screen out the most suitable medium for the introduced resource varieties (lines) SN6 and SN7 and self-breeding line YC24 of Cerasus humilis, and to determine the regeneration system of the three varieties, so as to provide technical support for the efficient and rapid large-scale propagation. Three C. humilis varieties were used as materials to study the disinfection methods of explants, the optimal medium for differentiation, proliferation and rooting and the ratio of hormones. The results showed that when the cephalosporin concentration was 100 mg/L, the explants had the highest survival rate and the lowest contamination rate. The optimal differentiation medium for SN6 and SN7 was MS+ 0.5 mg/L 6-BA+ 1.0 mg/L ZT+ 0.05 mg/L IBA, and the optimal differentiation medium for YC24 was MS+ 0.5 mg/L 6-BA+ 0.5 mg/L ZT+ 0.05 mg/L IBA. The optimal rooting medium of SN6 was 1/2MS+ 0.2 mg/L NAA+ 0.1 mg/L IBA+1.0 g/L activated carbon, and the optimal one-step rooting medium for SN7 and YC24 was 1/2MS+ 0.05 mg/L NAA+ 0.2 mg/L IBA. This study established a rapid propagation system for the shoots of C. humilis, and could lay a foundation for the subsequent transplanting, culture expanding and popularizing of the fine germplasms.

Key words: Cerasus humilis, endophytes, differentiation, rooting, cycocel, efficient regeneration

中图分类号: