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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (15): 118-126.doi: 10.11924/j.issn.1000-6850.casb2022-0486

• 资源·环境·生态·土壤 • 上一篇    下一篇

基于高通量测序分析陕西省苹果园土壤线虫群落分布特征

田稼1(), 李飞1, 孙超1, 上官亦卿2,3, 齐凡2,3()   

  1. 1 陕西省微生物研究所，西安 710043
    2 陕西省科学院，西安 710043
    3 中国科学院西安分院，西安 710043
  • 收稿日期:2022-06-15 修回日期:2022-08-15 出版日期:2023-05-25 发布日期:2023-05-22
  • 通讯作者: 齐凡，女，1964年生，陕西西安人，副研究员，学士，研究方向：微生物及生物化学。通信地址：710043 陕西省西安市咸宁中路125号 陕西省科学院 中国科学院西安分院，Tel：029-83282998，E-mail：qifan@xab.ac.cn。
  • 作者简介:

    田稼，男，1983年生，陕西西安人，副研究员，硕士，研究方向：农业微生物。通信地址：710043 陕西省西安市西影路76号 陕西省微生物研究所，Tel：029-82357019，E-mail：。

  • 基金资助:
    陕西省科学院科技计划项目“老龄苹果园土壤环境调查及微生物复混肥料的改良效果研究”(2015K-08); “陕西省苹果园土壤生态环境评价及改良土壤环境对果业发展战略意义研究”(2016K-09); 陕西省农业科技创新与攻关项目“改良老龄果园土壤的苹果专用微生物肥料研发”(2015NY027); 陕西省苹果研究发展项目“针对老龄果园土壤改良的苹果专用微生物复混肥研发”

Soil Nematode Communities in Apple Orchards in Shaanxi Province: Distribution Characteristics Analysis Based on High-throughput Sequencing

TIAN Jia1(), LI Fei1, SUN Chao1, SHANGGUAN Yiqing2,3, QI Fan2,3()   

  1. 1 Shaanxi Institute of Microbiology, Xi’an 710043
    2 Shaanxi Academy of Sciences, Xi’an 710043
    3 Chinese Academy of Sciences Xi’an Branch, Xi’an 710043
  • Received:2022-06-15 Revised:2022-08-15 Online:2023-05-25 Published:2023-05-22

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摘要：

为了明确陕西省苹果园土壤线虫分布特征。本研究以3个产业带179个果园土壤总DNA为材料，采用高通量测序技术分析了果园土壤线虫群落组成。结果表明，陕西省苹果园土壤包含线虫2纲，12目，93属，其中Hexamermis是优势属，分别占陕北山地、渭北北部和渭北南部产业带的92.20%，89.73%和88.63%。不同产业带（纬度）和海拔对线虫ACE和Shannon指数具有显著影响(P<0.05)。聚类分析表明，渭北北部和渭北南部产业带线虫在属水平分布更相近。土壤含水率、有机质、全氮、全磷及pH与土壤线虫多样性指数及优势属Hexamermis和Paratylenchus显著相关(P<0.05)。本研究表明陕西省苹果园土壤线虫组成较丰富，但优势属较少。除纬度和海拔外，改良土壤因子对优化线虫群落结构，进而改善果园土壤环境具有重要作用。

关键词: 土壤线虫, 分布特征, 高通量测序, 苹果园, 黄土高原

Abstract:

The aim is to elucidate the distribution characteristics of soil nematodes in apple orchards in Shaanxi Province. This study analyzed the composition of soil nematode communities by high-throughput sequencing, using total soil DNA from 179 orchards in three industrial belts. The results show that there are two classes, 12 orders, and 93 genera of soil nematodes in the apple orchards in Shaanxi Province, of which Hexamermis is the dominant genus, accounting for 92.20%, 89.73%, and 88.63% in the mountainous region in northern Shaanxi, northern Weibei and southern Weibei industrial belt, respectively. The ACE and Shannon indexes of nematodes are significantly affected by different industrial belts (latitudes) and altitudes (P<0.05). The cluster analysis shows that the distribution of nematodes in the northern Weibei industrial belt is more similar to that in the southern Weibei industrial belt at the genus level. Soil moisture content, organic matter, total nitrogen, total phosphorus, and pH are significantly correlated with soil nematode diversity indexes and dominant genera, such as Hexamermis and Paratylenchus (P<0.05). This study reveals that soil nematodes are abundant in the apple orchards in Shaanxi Province, but the dominant genera are fewer. In addition to latitude and altitude, improving soil properties can play a crucial role in optimizing the nematode community structure, and subsequently the soil environment in the apple orchards.

Key words: soil nematode, distribution characteristic, high-throughput sequencing, apple orchard, Loess Plateau