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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (13): 109-116.doi: 10.11924/j.issn.1000-6850.casb2022-0396

• 植物保护·农药 • 上一篇    下一篇

菊花枯萎病植株根际土壤细菌群落多样性比较

赵娅红1(), 吴治兴1, 刘敏荣3, 涂艳芳2, 薛建平3, 王悦1, 卢超1, 刘佳妮1, 余磊1, 姚茹瑜4, 陈志星3(), 黄飞燕1()   

  1. 1 昆明学院农学与生命科学学院/云南省都市特色农业工程技术研究中心，昆明 650214
    2 开远市农业技术推广中心，云南红河 661600
    3 昆明虹之华园艺有限公司，昆明 651700
    4 云南农业职业技术学院，昆明 650031
  • 收稿日期:2022-05-18 修回日期:2022-11-10 出版日期:2023-05-05 发布日期:2023-04-27
  • 通讯作者: 黄飞燕，女，1984年出生，四川德阳人，副研究员，博士，主要从事作物遗传育种研究。通信地址：650214 云南省昆明市官渡区浦新路2号昆明学院，E-mail：125593879@qq.com。陈志星，男，1982年出生，山西大同人，高级农艺师，硕士，主要从事植物病虫害研究。通信地址：651708 云南省昆明市嵩明县小街省花卉示范园区，E-mail：czx-717@163.com。
  • 作者简介:

    赵娅红，女，1991年出生，云南昆明人，在读硕士，主要从事作物健康栽培研究。通信地址：650214 云南省昆明市官渡区浦新路2号昆明学院农学楼，E-mail：。

  • 基金资助:
    云南省科技重点研发计划项目“菊花新品种选育与高效生产技术集成”(2018BB011); 昆明市科技计划项目“昆明市昆明虹之华园艺有限公司科技创新团队及企业科技创新中心”(2022-RCC-CXTD-3); 昆明市科技计划项目“昆明市昆明虹之华园艺有限公司科技创新团队及企业科技创新中心”(2022-CGC-CXZX-3); 云南省教育厅科学研究基金项目“黑胫病菌胁迫下烟草microRNA的高通量测序及生物信息学分析”(2022Y708)

Bacterial Community Diversity in Rhizosphere Soil of Chrysanthemum Wilt Plants: A Comparative Study

ZHAO Yahong1(), WU Zhixing1, LIU Minrong3, TU Yanfang2, XUE Jianping3, WANG Yue1, LU Chao1, LIU Jiani1, YU Lei1, YAO Ruyu4, CHEN Zhixing3(), HUANG Feiyan1()   

  1. 1 College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214
    2 Kaiyuan Agricultural Technology Promotion Center, Honghe, Yunnan 661600
    3 Kunming Hongzhihua Horticulture Co., Ltd., Kunming 651700
    4 Yunnan Agricultural Vocational and Technical College, Kunming 650031
  • Received:2022-05-18 Revised:2022-11-10 Online:2023-05-05 Published:2023-04-27

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摘要：

菊花枯萎病 (Fusarium oxysporum f. sp. chrysanthemi)是目前影响菊花产业发展的一种侵入土壤根部传播的病害。笔者利用Illumina MiSeq高通量测序技术探讨菊花患枯萎病的发病植株和菊花健康植株的土壤根际微生物群落结构的差异。结果表明，菊花枯萎病的发生对根际土菌落丰富度存在明显影响。健康菊花植株和患病菊花植株根际细菌OTU数分别是1831和1723个，两者共有为1661个，菊花健株根际土壤中细菌OTU总数比菊花病株增加6.23%，特有OTUs数是患病植株的2.74倍。从土壤根际群落结构的多样性水平上看，菊花患枯萎病植株低于菊花健康植株。Alpha多样性指数显示，菊花病株根际土壤的Shannon、Ace以及Chao1指数比健株降低1.60%、5.71%和6.62%。此外，菊花患枯萎病植株与健康植株在土壤细菌群落多样性的结构上也有明显差异，菊花健康植株根际土中，主要α-变形菌纲(α-proteobacteria)、芽孢杆菌纲(Bacilli)、绿弯菌纲(Chloroflexia)，其相对丰富度分别比病株高10.27%、1.51%、9.54%；在属水平上，病株根际土中芽孢杆菌属(Bacillus)、鞘氨醇单胞菌属(Sphingomonas)、节杆菌属(Arthrobacter)相对丰富度比健株分别低4.14%、0.45%、39.24%。上述结果说明，在菊花根际土中，细菌群落结构的改变以及物种多样性的降低是菊花枯萎病发生的关键因素，研究结果可为更早防治菊花枯萎病提供理论依据。

关键词: 菊花, 根际土壤, 高通量测序, 微生物, 细菌群落

Abstract:

Chrysanthemum wilt (Fusarium oxysporum f. sp. chrysanthemi) is a root-invading disease that affects the development of chrysanthemum industry. In this study, Illumina MiSeq high-throughput sequencing was used to analyze the differences in rhizosphere soil microbial community structure between wilt-affected plants and healthy plants of chrysanthemum. The results showed that the incidence of chrysanthemum wilt had a significant impact on the abundance of the bacterial community in rhizosphere soil. The number of bacterial OTUs in the rhizosphere of healthy chrysanthemum plants and diseased chrysanthemum plants were 1831 and 1723, respectively, with a share of 1661. The total number of bacterial OTUs in the rhizosphere soil of healthy chrysanthemum plants was 6.23% higher than that of diseased plants, and the number of unique OTUs was 2.74 times that of diseased plants. From the diversity level of soil rhizosphere bacterial community structure, chrysanthemum wilt plants were lower than chrysanthemum healthy plants. Alpha diversity index showed that Shannon, Ace and Chao1 indexes of rhizosphere soil of chrysanthemum wilt plants were 1.60%, 5.71% and 6.62% lower than those of healthy plants, respectively. In addition, chrysanthemum wilt plants and chrysanthemum healthy plants also had significant differences in the structure of soil bacterial community diversity. In the rhizosphere soil of chrysanthemum healthy plants, the relative abundance of main α-proteobacteria, Bacilli, Chloroflexia were 10.27%, 1.50% and 9.54% higher than those of diseased plants, respectively. At the genus level, the relative abundance of genus Bacillus, Sphingomonas and Arthrobacter in the rhizosphere soil of diseased plants were 4.14%, 0.45% and 39.24% lower than those of healthy plants, respectively. The above results indicate that in the rhizosphere soil of chrysanthemum, the change of bacterial community structure and the reduction of species diversity are the key factors for the incidence of chrysanthemum wilt. This study will provide a theoretical basis for the control of chrysanthemum wilt.

Key words: chrysanthemum, rhizosphere soil, high-throughput sequencing, microorganism, bacterial community