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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (6): 116-123.doi: 10.11924/j.issn.1000-6850.casb2022-0348

所属专题： 生物技术； 植物保护

• 植物保护·农药 • 上一篇    下一篇

茶饼病病叶表面微生物多样性及病害真菌的分离鉴定

陈义勇1(), 周波1, 黎健龙1, 唐颢1, 崔莹莹1, 吴文浩2, 刘嘉裕1, 唐劲驰1()   

  1. 1 广东省农业科学院茶叶研究所，广东省茶树资源创新利用重点实验室，广州 510640
    2 华南农业大学园艺学院，广州 510640
  • 收稿日期:2022-05-10 修回日期:2022-08-27 出版日期:2023-02-25 发布日期:2023-02-22
  • 通讯作者: 唐劲驰，女，1973年出生，山西阳泉人，研究员，博士，主要从事茶树营养与茶树生态栽培研究。通信地址：510640 广东省广州市天河区大丰路6号 广东省农业科学院茶叶研究所，Tel：020-85283530，E-mail：tangjinchi@126.com。
  • 作者简介:

    陈义勇，男，1989年出生，湖北荆门人，副研究员，博士，研究方向：茶树病害与茶叶品质。通信地址：510640 广东省广州市天河区大丰路6号 广东省农业科学院茶叶研究所，Tel：020-38797942，E-mail：。

  • 基金资助:
    广东省基础与应用基础研究基金“茶饼病菌(Exobasidium vexans)感染茶树叶片过程与茶树的互作机制研究”(2020A1515011344); 广东省农业科学院农业优势产业学科团队建设项目“新兴学科团队-茶园精准管控栽培”(202125TD); 广东省农业科学院人才项目“优秀博士”(R2017YJ-YB3015); “青年导师制”(R2018QD-102); “青年副研究员”(R2019PY-QF002); 广西重点研发计划“岑溪野生茶树种质资源的创新与应用”（桂科AB18294025(桂科AB18294025)

Blister Blight Lesions of Tea (Camellia sinensis L. Kuntze) Leaves: Microbial Diversity Analysis and Identification of the Disease Fungi

CHEN Yiyong1(), ZHOU Bo1, LI Jianlong1, TANG Hao1, CUI Yingying1, WU Wenhao2, LIU Jiayu1, TANG Jinchi1()   

  1. 1 Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou 510640
    2 College of Horticulture, South China Agricultural University, Guangzhou 510640
  • Received:2022-05-10 Revised:2022-08-27 Online:2023-02-25 Published:2023-02-22

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摘要：

为了分析茶饼病病叶表面微生物多样性，分离鉴定病叶上真菌微生物。以茶饼病病叶与健康叶片为试材，采用扫描电镜对叶表面进行观察，利用16S和ITS测序分析不同叶片的细菌与真菌群落组成。在茶饼病病害叶片表面检测到113种细菌，252种真菌；健康叶片上检测到237种细菌，161种真菌。病叶细菌的多样性显著低于健康叶片，病叶真菌的多样性高于健康叶片。与健康叶片相比，枝孢属和拟盘多毛孢属真菌在病叶中丰度较低，被孢霉属丰度较高。采用PDA培养基从感病叶片表面分离鉴定获得了枝状枝孢菌，该菌可能在茶饼病的感染过程中发挥着重要作用。茶饼病感染后茶叶表面微生物群落结构发生显著变化，分离鉴定出的枝状枝孢菌可能与茶饼病的致病过程密切相关。

关键词: 茶饼病, 茶, 微生物多样性, 细菌, 真菌

Abstract:

The aims are to analyze the microbial diversity on the surface of tea blister blight diseased leaves, and isolate and identify fungal microorganisms on diseased tea leaves. Tea leaves infected with blister blight disease and healthy leaves were used as test materials, and the leaf surface was observed by scanning electron microscopy, and the bacterial and fungal community compositions of different leaves were analyzed by 16S and ITS sequencing. 113 kinds of bacteria and 252 kinds of fungi were detected on the surface of tea blister blight diseased leaves, and 237 kinds of bacteria and 161 kinds of fungi were detected on healthy leaves. The bacterial diversity of diseased leaves was significantly lower than that of healthy leaves, and the fungi diversity of diseased leaves was higher than that of healthy leaves. Compared with healthy leaves, the abundance of Cladosporium and Pestalotiopsis in diseased leaves was lower, while that of Mortierella was higher. Fungal microorganism Cladosporium cladosporioides was isolated by PDA medium and identified, which might play an important role in the infection of blister blight disease. The microbial community structure on the surface of tea leaves changed significantly after tea blister blight disease infection, and the isolated and identified Cladosporium cladosporium might be closely related to the pathogenic process of tea blister blight disease.

Key words: blister blight, Camellia sinensis, microbial diversity, bacteria, fungi