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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (6): 124-128.doi: 10.11924/j.issn.1000-6850.casb2022-0185

所属专题： 生物技术； 植物保护

• 植物保护·农药 • 上一篇    下一篇

青海高寒草地牧草拮抗内生细菌的筛选、鉴定及促生功能测定

尧贺敏(), 汤梅, 巩雪花, 金梦军, 杨成德()   

  1. 甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室，兰州 730070
  • 收稿日期:2022-03-13 修回日期:2022-04-12 出版日期:2023-02-25 发布日期:2023-02-22
  • 通讯作者: 杨成德，男，1975年出生，甘肃武都人，教授，博士研究生，博士，主要从事植物病理学等方面工作。通信地址：730070 甘肃省兰州市安宁区营门村1号 甘肃农业大学植物保护学院，Tel：0931-7640744，E-mail：yangcd@gsau.edu.cn。
  • 作者简介:

    尧贺敏，女，2000年出生，本科，植物病理学方向。通信地址：730070 甘肃省兰州市安宁区营门村1号 甘肃农业大学植物保护学院，Tel：17779123052，E-mail：。

  • 基金资助:
    甘肃农业大学国家级大学生创新创业训练计划项目(202019017); 国家自然科学基金“东祁连山高寒草地牧草内生细菌的多样性及其与宿主互作对逆境胁迫的响应”(31660148)

Screening, Identification and Functional Determination of Endophytic Bacteria from Forage Grass at Alpine Grassland in Qinghai Province

YAO Hemin(), TANG Mei, GONG Xuehua, JIN Mengjun, YANG Chengde()   

  1. College of Plant Protection of Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu, Lanzhou 730070
  • Received:2022-03-13 Revised:2022-04-12 Online:2023-02-25 Published:2023-02-22

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摘要：

以尖镰孢菌黄瓜专化型(Fusarium oxysporum f.sp.cucumerium)为指示菌从青海高寒草地牧草内生细菌中筛选拮抗菌株，结合形态学特征和16Sr DNA基因序列同源性分析鉴定，并进行促生功能测定。结果表明，供试的81株内生细菌中36株有拮抗作用，占供试菌株的44.0%，其中从根部分离的13株的抑菌率均在50.0%以上，菌株2018GH11对6属8种病原真菌的抑菌率均在50.0%以上，特别对孜然根腐病菌的抑菌率达67.6%，抑菌谱宽；根据形态学特征和16Sr DNA基因序列分析将其鉴定为暹罗芽孢杆菌Bacillus siamese；2018GH11具有固氮能力，无溶磷和产IAA能力；发酵菌剂2018GH11用量为0.50%时，黄瓜株高、茎粗、叶片数、鲜重及干重分别比对照增加了15.33%、39.40%、22.22%、215.99%和126.70%。该结果表明高寒草地牧草内生细菌2018GH11具有良好的防病促生作用，开发潜力巨大。

关键词: 尖镰孢菌黄瓜专化型, 内生细菌, 拮抗, 筛选, 鉴定

Abstract:

The antagonistic strains against Fusarium oxysporum f.sp.cucumerium were screened from endophytic bacteria of forage grass at alpine grassland in Qinghai Province, and then were identified by morphological characteristics and homology analysis of 16Sr DNA gene sequence, and their biological functions were determined. The results showed that 36 strains of the 81 tested endophytic bacteria strains had antagonistic effects, accounting for 44.0%, and the inhibition rate of 13 strains isolated from the root were all above 50.0%, among which the inhibition rate of 2018GH11 was above 50.0% against 6 genera 8 species of pathogenic fungi, and 67.6% against Fusarium oxysporum. 2018GH11 had a broad spectrum of inhibition, and it was identified as Bacillus siamese by the morphological characteristics and homology analysis of 16Sr DNA gene sequence. 2018GH11 had nitrogen fixation ability, but the ability to produce IAA and solubilize phosphate was not obvious. In pot experiment, when the amount of 2018GH11 was 0.50%, the cucumber height, stem diameter, leaf number, fresh weight and dry weight were increased by 15.33%, 39.40%, 22.22%, 215.99% and 126.70%, respectively, compared with those of the control plants. In summary, the endophytic bacteria 2018GH11 from alpine grassland has a good effect on controlling disease and promoting plant growth, and a great potential for development.

Key words: Fusarium oxysporum f.sp.cucumerium, endophytic bacteria, antagonism, screening, identification