欢迎访问《中国农学通报》,

中国农学通报 ›› 2023, Vol. 39 ›› Issue (2): 123-129.doi: 10.11924/j.issn.1000-6850.casb2022-0066

所属专题: 水产渔业

• 水产·渔业 • 上一篇    下一篇

克氏原螯虾微小杆菌Exiguobacterium profundum的分离鉴定与药敏试验

石瑞雪(), 李艳和()   

  1. 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室,武汉 430070
  • 收稿日期:2022-02-11 修回日期:2022-09-26 出版日期:2023-01-15 发布日期:2023-01-17
  • 作者简介:

    石瑞雪,女,1997年出生,硕士研究生,研究方向:水产养殖。通信地址:430070 湖北省武汉市洪山区狮子山街1号,E-mail:

  • 基金资助:
    中央高校基本科研业务费专项资金资助项目“克氏原螯虾白斑综合征抗病选育关键技术研究”(2662020SCPY004); 国家自然科学基金青年基金项目“基于GBS技术的克氏原螯虾系统地理学研究”(31501858)

Isolation, Identification and Antibiotics Susceptibility Test of Exiguobacterium profundum from Procambarus clarkii

SHI Ruixue(), LI Yanhe()   

  1. College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070
  • Received:2022-02-11 Revised:2022-09-26 Online:2023-01-15 Published:2023-01-17

摘要:

为了判断克氏原螯虾(Procambarus clarkii)发病致死的原因,从发病濒死虾肝胰脏中分离出一株优势菌,通过对该菌进行菌落形态观察、革兰氏染色、生理生化鉴定、16srRNA测序分析及进化树构建确认其为微小杆菌Exiguobacterium profundum。通过纸片扩散法测定了该菌株对青霉素G、氨苄西林、头孢氨苄、阿米卡星、庆大霉素、卡那霉素、诺氟沙星、复方新诺明等30种抗菌药物的敏感性,结果显示该菌对30种药物均敏感。通过回归感染实验推测其可能参与克氏原螯虾的致病过程。本实验结果为克氏原螯虾E. profundum的病原鉴定和药物诊治提供了一定的参考和理论基础。

关键词: 克氏原螯虾, 微小杆菌Exiguobacterium profundum, 病原鉴定, 16srRNA基因, 药敏试验, 回归感染

Abstract:

To identify the disease and mortality cause of Procambarus clarkii, a dominant strain isolated from hepatopancreas of the dying crayfish was identified as Exiguobacterium profundum by colony morphology observation, Gram staining, physiological and biochemical identification, 16srRNA sequencing analysis and phylogenetic tree construction. The resistance of this strain to 30 kinds of antibacterial drugs, including mycin G, ampicillin, cephalexin, amikacin, gentamicin, kanamycin, norfloxacin, cotrimoxazole and so on, was determined by disc diffusion method, and the results showed that the strain was sensitive to all the 30 kinds of drugs. After the regression infection experiment, it was inferred that the strain might be involved in the pathogenic process of P. clarkii. The current study could provide certain reference and a theoretical basis for the pathogen identification, drug diagnosis and treatment of E. profundum in P. clarkii.

Key words: Procambarus clarkii, Exiguobacterium profundum, pathogen identification, 16srRNA gene, drug susceptibility test, regression infection experiment