中国农学通报 ›› 2022, Vol. 38 ›› Issue (3): 87-93.doi: 10.11924/j.issn.1000-6850.casb2021-0316
所属专题: 生物技术
陈柳宏1,2(), 赵春雷1,3, 王希1,3, 李彦丽1,3,4, 丁广洲1,2(
), 陈丽1,3(
)
收稿日期:
2021-03-26
修回日期:
2021-07-08
出版日期:
2022-01-25
发布日期:
2022-02-25
通讯作者:
丁广洲,陈丽
作者简介:
陈柳宏,女,1998年出生,四川达州人,在读硕士,研究方向:甜菜分子育种与种质资源,参与黑龙江省经济作物产业技术协同创新体系甜菜育种岗位科学家项目、国家糖料产业技术体系(甜菜品种资源评价)岗位科学家项目等。通信地址:150080 黑龙江大学现代农业与生态环境学院,Tel:18702256452,E-mail: 基金资助:
CHEN Liuhong1,2(), ZHAO Chunlei1,3, WANG Xi1,3, LI Yanli1,3,4, DING Guangzhou1,2(
), CHEN Li1,3(
)
Received:
2021-03-26
Revised:
2021-07-08
Online:
2022-01-25
Published:
2022-02-25
Contact:
DING Guangzhou,CHEN Li
摘要:
为探究单细胞转录组测序技术(scRNA-seq)在植物研究中的应用,本综述对单细胞转录组测序技术的发展状况进行了总结,介绍了其在胚胎发育、免疫细胞、肿瘤领域以及植物研究的运用。其次,就单细胞转录组测序技术中的要点技术(单细胞分离技术、单细胞全转录组cDNA扩增技术、高通量测序技术)进行了总结分析。最后,本文根据植物细胞的特点归纳出植物所适用的单细胞转录组测序技术,以期为该技术在植物研究中更好的利用提供理论依据。
中图分类号:
陈柳宏, 赵春雷, 王希, 李彦丽, 丁广洲, 陈丽. 单细胞转录组测序技术及其在植物研究中的应用[J]. 中国农学通报, 2022, 38(3): 87-93.
CHEN Liuhong, ZHAO Chunlei, WANG Xi, LI Yanli, DING Guangzhou, CHEN Li. Single-cell Transcriptome Sequencing Technology and Its Application in Plant Research[J]. Chinese Agricultural Science Bulletin, 2022, 38(3): 87-93.
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