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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (2): 137-142.doi: 10.11924/j.issn.1000-6850.casb2023-0304

• 食品·营养·检测·安全 • 上一篇    下一篇

红甜菜根中萜类代谢物的比较代谢组学分析

李佳颖1(), 刘乃新1(), 韩广源2   

  1. 1 黑龙江大学现代农业与生态环境学院，哈尔滨 150080
    2 哈尔滨海关技术中心，哈尔滨 150023
  • 收稿日期:2023-02-23 修回日期:2023-05-15 出版日期:2024-01-10 发布日期:2024-01-10
  • 通讯作者:
    刘乃新，女，1980年出生，黑龙江哈尔滨人，副研究员，研究方向：糖料作物质量安全及营养品质评价。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学农学楼，E-mail：。
  • 作者简介:

    李佳颖，女，2003年出生，广西桂林人，本科，研究方向：植物代谢组学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学农学楼，E-mail：。

  • 基金资助:
    财政部和农业农村部国家现代农业产业技术体系（糖料）项目(CARS-170503); 黑龙江省博士后基金项目“纳米硒生物强化调控寒地香稻产量和品质的分子机制研究”(lbh-z22245)

Comparative Metabolomics Analysis of Terpenoid Metabolites in Root of Red Beet

LI Jiaying1(), LIU Naixin1(), HAN Guangyuan2   

  1. 1 College of Modern Agriculture and Eco-Environment, Heilongjiang University, Harbin 150080
    2 Harbin Customs Technology Center, Harbin 150023
  • Received:2023-02-23 Revised:2023-05-15 Published-:2024-01-10 Online:2024-01-10

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摘要：

为了比较不同红甜菜品种间萜类代谢物差异，进一步创制红甜菜新种质，本研究采用UPLC-MS/MS检测平台和Metware自建数据库MWDB(Metware database)对俄罗斯（红甜菜）、一品红（红甜菜）进行广泛靶向代谢组学分析，结合聚类和多元统计等多种统计分析方法筛选差异代谢物， 研究结果表明：在两组红甜菜块根样品中共检测出48种萜类物质，分为倍半萜、单萜、二萜、三萜、三萜皂苷和其他萜类共六大类，其中倍半萜13种，单萜7种，二萜13种，三萜7种，三萜皂苷7种，以及布卢门醇C。在两组样品中筛选出的差异代谢物8种，其中下调物质5种，分别为桃叶珊瑚苷、苜蓿酸-3-O-葡萄糖醛酸苷-28-O-鼠李糖基(1,2)-阿拉伯糖苷、6'-O-芥子酰京尼平苷、筋骨草苷和原莪术醇；上调的物分别为Cis-guai-10-en-4α,11-diol、6'-O-葡萄糖桃叶珊瑚苷和6,9-二羟基-7-巨豆烯-3-酮。不同品种红甜菜中存在显著差异的萜类代谢物，可作为特征代谢物做进一步分析。

关键词: 红甜菜, 根, 萜类物质, 差异代谢, 代谢组学

Abstract:

In order to compare the differences in terpenoid metabolites between different red beet varieties and further create new red beet germplasm, this study used the UPLC-MS/MS detection platform and MWDB (Metware database) to conduct extensive targeted metabolomic analyses of Russian (red beet) and poinsettia (red beet), and combined statistical analysis methods such as clustering and multivariate statistics to screen differential metabolites. The results showed that a total of 48 terpenoids were detected in the two groups of red beet root samples. There were six categories of sesquiterpenes, monoterpenes, diterpenes, triterpenes, triterpene saponins, and other terpenes, including 13 kinds of sesquiterpenes, 7 kinds of monoterpenes, 13 kinds of diterpenes, 7 kinds of triterpenes, and 7 kinds of triterpene saponins, and blumentol C. 8 differential metabolites were screened out in the two groups of samples, of which 5 were down-regulated, including aucubin and alfalfa-3-O-glucuronide-28-O-rhamnosyl (1, 2)-arabinopyranoside, 6'-O-sinapoyl geniposide, mycoside, and protocurcumol; the upregulated substances were Cis-guai-10-en-4α,11-diol, 6'-O- Glucose aucubin and 6,9-dihydroxy-7-sodium-3-ketone. There were significantly different terpene metabolites in different red beet varieties, which could be used as characteristic metabolites for further analysis.

Key words: red beets, root, terpenes, differential metabolites, metabolomics