采后果蔬病害生物防治研究进展

摘要/Abstract

摘要： 综述了近十年果蔬采后病害生物防治的研究进展；利用天然植物产物、混合菌剂防治采后病害以及拮抗菌活体筛选是研究的热点。活体筛选出的拮抗微生物容易定殖，适应性强；混合菌剂能减少化学药剂使用量且防治效果好。概括了常用的几种拮抗菌的筛选方法并阐述了拮抗菌生防机制的研究现状。

关键词: 农民参与式, 农民参与式, 木薯, 农业科技成果, 转化推广

Abstract: This article provides an overview of the biocontrol on the post-harvest diseases of garden stuff in recent decades. Researches of utilization of natural plant products, mixed-agents and screening antagonists in vivo have become hot topics. Antagonists from in-vivo-screening are adaptable and can easily colonize the plants; Mixed-agents can reduce the dosage of fungicides, and, control the diseases well. Several common methods to screen antagonists are summarized and the bio-control mechanisms are described in this paper.

中图分类号:

S432

邱逸斯,于莉. 采后果蔬病害生物防治研究进展[J]. 中国农学通报, 2006, 22(9): 351-351.

Qiu Yisi, Yu li. Recent Advances on the Bio-control of Postharvest Diseases[J]. Chinese Agricultural Science Bulletin, 2006, 22(9): 351-351.

参考文献

1 田大业.保鲜产业大有可为.中小企业科技,2005,(1):17
2 盛爱武,乔爱民,高飞,等.富贵竹切口的保鲜及贮运研究.园艺园林科学,2005,21(9):329~331
3 胡美姣,刘秀娟,张令宏.热带亚热带水果采后病害的生物防治.热带农业科学,2001,4(2):51~59
4 Wilson C L, Solar J M, El Ghaouth A El ,et al. Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea.Plant Dis,1997,81:204~210
5 Negi P S, Jayaprakasha G K. Antibacterial activity of grapefruit (Citrus paradisi) peel extracts.Eur Food Res Technol,2001,213:484~487
6 孔秋莲,张明融,章丽丽.中草药提取物对两种果蔬致腐真菌的抑制作用.保鲜与加工,2002,(3):17~18
7 蒋继志,史娟,赵丽坤,等.几种植物提取物诱导马铃薯对致病疫霉的抗性.植物病理学报,2001,31(2):145~151
8 Wilson C L, Wisniewski M E, Droby S, et al. A selection strategy for microbial antagonists to control postharvest diseases of fruits and vegetables.Scientia Horticulturae, 1993,53:183~189
9 杜红方.棉花黄萎病拮抗细菌的筛选与抗菌蛋白的分离纯化:[学位论文].河北:河北大学,2003.21
10 郑爱萍,李平,王世全,等.水稻纹枯病菌强拮抗菌B34的分离与鉴定.植物病理学报,2003,33(1):81~85
11 徐增富,邱国华,王金发,等.水稻白叶枯病菌的拮抗菌的筛选及其抗菌物质的研究.中山大学学报
（自然科学版）1994,33(3):122~124
12 范青,田世平,姜爱丽,等.采摘后果实病害生物防治拮抗菌的筛选和分离.中国环境科学,2001,21(4):313~316
13 范青,田世平,李永兴,等.枯草芽孢杆菌(Bacillus subtilis)B-912对采后柑桔果实青、绿霉病的抑制效果.植物病理学报,2000,30(4):343~348
14 Zhou Ting, Chu ChunLung, Liu Wei T, et al. Postharvest control of blue mold and gray mold on apples using isolates of Pseudomonas syringae. Canadian Journal of Plant Pathology, 2001, 23(3):246~252
15 Janisiewicz W J, Jeffers S N. Efficacy of commercial formulation of two biofungicides for control of blue mold and gray mold of apples in cold storage.Crop Protection,1997,16(7):629~633
16 Shachnai A,Chalutz E, Droby S, et al. Commercial use of AspireTM for the control of postharvest decay of Citrus fruit in the packing houses.Proceedings of the International Society of Citriculture,1996,(2):1174~1177
17 Laura A Silo-Suh, Benjamin J Lethbridge, Sandra J Raffel, et al. Biological Activities of Two Fungistatic Antibiotics Produced by Bacillus cereus UW85.Applied and Environmental Microbiology,1994,60(6):2023~2030
18 Leifert C, Li H, Chidburee S, Hampson S, et al. Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45.Appl Bacteriol,1995,78(29):97~108
19 Han J S, Cheng J H, Yoon T M, et al. Biological control agent of common scab disease by antagonistic strain Bacillus sp. sunhua. Journal of Applied Microbiology,2005, 99:213~221
20 Maget-Dana R, Ptak M, Peypoux F, Michel G. Poreforming properties of iturin A,a lipopeptide antibiotic. Biochim Biophys Acta, 1985, 815:405~409
21 Santos A, Marquina D. Killer toxin of Pichia membranifaciens and its possible use as a biocontrol agent against grey mould disease of grapevine.Microbiology,2004,150:2527~2534
22 Wisniewski M, Biles C, Droby S, et al. Mode of action of the postharvest biocontrol yeast,Pichia guililiermondii.I. Characterization of attachment to Botrytis cinerea. Physiological and Molecular PlantPathol,1991,39:245~258
23 Nielsen T. H., S?rensen D, Tobiasen C. et al. Antibiotic and Biosurfactant Properties of Cyclic Lipopeptides Produced by Fluorescent Pseudomonas spp. from the Sugar Beet Rhizosphere. Applied and Environmental Microbiology, 2002,68(7):3416~3423
24 Zhan Yuan, Song Cang, Matsufuji M, et al. High Production of Pyoluteorin and 2,4-Diacetylphloroglucinol by Pseudomonas fluorescens S272 Grown on Ethanol as a Sole Carbon Source. Journal of Fermentation and Bioengineering, 1998,86(6):559~563
25 Maurhofer M, Keel C, Haas D, et al. Pyoluteorin production by Pseudomonas fluorescens strain CHA0 is involved in the suppression of Pythium damping-off of cress but not of cucumber. European Journal of Plant Pathology, September 1994: 221~232
26 De Marco J L, Lima LHC, valle de Sousa M, et al. A Trichoderma harzianum chitinase destroys the cell wall of the phytopathogen Crinipellis perniciosa, the casual agent of witches’ broom disease of cocoa. World Journal of Microbiol & Biotechnol,2000,16: 383~386
27 Viterbo Ada, Ramot Ofir, Chernin Leonid, et al. Significance of lytic enzymes from Trichoderma spp. in the biocontrol of fungal plant pathogens.Antonie van Leeuwenhoek, 2002, 81: 549~556
28 Elad Y, Kapat A. The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea. European Journal of Plant Pathology,1999, 105: 177~189
29 Weindling, R. Trichoderma lignorum as a parasite of other soil fungi. Phytopathology, 1932, 22:837~845
30 Arras G. Mode of action of an isolate of Candida famata in biological control of Penicillium digitatum in orange fruits.Postharvest Biology and Technology,1996,8:191~198
31 Gupta V P, Tewari S K, Govindaiah, et al. Ultrastructure of mycoparasitism of Trichoderma, Gliocladium and Laetisaria species on Botryodiplodia theobromae.Journal of Phytopathology,Jan 1999, 147(1):19~24
32 Castoria R, De Curtis F, Lima G, et.al. β-1,3-glucanase activity of two saprophytic yeast and possible mode of action as biocontrol agents against postharvest diseases.Postharvest Biol Technol,1997,12:293~300
33 乔宏萍.重寄生放线菌F46和PR的生物学习性及对果、蔬采后病害的控制作用:[学位论文].中国杨凌:西北农林科技大学,2003.9
34 Wan Y K, Tian S P. Antagonistical Mode of Pichia membrane faciens to Rhizopus Stolonifer in Wounds of Peach Fruit.Acta Botanica Sinica,2002,44(11):1384~1386
35 梁泉峰,池振明.间型假丝酵母菌株对多种水果蔬菜腐败霉菌的拮抗效果和拮抗机制的研究.食品与发酵工业,2001,28(1):34~38
36 张红印.罗伦隐球酵母对水果采后病害的生物防治及其防治机理研究:[学位论文].浙江:浙江大学,2004.78~80
37 Ippolito A, Ghaouth A E, Wilson C L, Wisniewski M. Control of postharvest decay of apple fruit by Aureobasidium pullulans and induction of defense responses.Postharvest Biology and Technology,2000,19:265~272
38 Piano S, Neyrotti V, Migheli Q, et al. Biocontrol capability of Metschnikowia pulcherrima against Botrytis postharvest rot of apple.Postharvest Biology and Technology,1997,11:131~140.
39 Liesbet Poppe, Sofie Vanhoutte, Monica H¨ofte. Modes of action of Pantoea agglomerans CPA-2,an antagonist of postharvest pathogens on fruits.European Journal of Plant Pathology, 2003,109: 963~973
40 Janisiewicz W J, Usall J. Nutritional Enhancement of Biocontrol of Blue Mold on Apples .Phytopathology, 1992.82:1364~1370
41 Droby S, Vinokur V, Weiss B, et al. Induction of resistance to Penicillium digitatum in grapefruit by the yeast biocontrol agent Candida oleophila.Phytopathology,2002,92:393~399
42 El-Ghaouth A, Wilson C L, Wisniewski M. Ultrastructural and cytochemical aspects of the biological control of Botrytis cinerea by Candida saitoana in apple fruit. Phytopathology,1998,88:282~291
43 杨冬梅.芽孢杆菌B1对“银帝”甜瓜主要采后病害的控制:[学位论文].甘肃:甘肃农业大学学位论文，2004.21
44 秦国政,田世平,刘海波,等.拮抗菌与病原菌处理对采后桃果实多酚氧化酶、过氧化物酶及苯丙氨酸解氨酶的诱导.中国农业科学,2003,36(1):89~93
45 Saskia CM Van Wees, Corné M J Pieterse, Annemiek Trijssenaar, et al. Differential Induction of Systemic Resistance in Arabidopsis by Biocontrol Bacteria.MPMI,1997,10(6):716~724
46 Janisiewicz W. Ecological diversity, niche overlap, and coexistence of antagonists used in developing mixtures for biocontrol of postharvest diseases of apples.Phytopathology,1996,86(5): 473~479
47 Guetsky R., Shtienberg D, Elad Y, et al. Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression.Phytopathology,2002,92:976~985
48 Janisiewicz W J. Enhancement of Biocontrol of Blue Mold with the Nutrient Analog 2-Deoxy-D-Glucose on Apples and Pears.Applied and Environmental Microbiology,1994, 60(8):2671~2676
49 Qin G Z, Tian S P. Enhancement of biocontrol activity of Cryptococcus laurentii by silicon and the possible mechanisms involved.Phytopathology,2005,95:69~75
50 Deena Errampalli, Nichole R Brubacher. Biological and integrated control of postharvest blue mold (Penicillium expansum) of apples by Pseudomonas syringae and cyprodinil, Biological ontrol,2006,36,49~56

[1]	刘子凡, 苏必孟, 黄洁, 魏云霞, 肖子丽. 木薯花生不同间作模式对木薯地土壤肥力的影响[J]. 中国农学通报, 2022, 38(25): 102-107.
[2]	赵大伟, 宋记明, 刘炜林, 孟凡来, 邓国军, 黄兴粉. 不同生态环境木薯高产高效栽培分析[J]. 中国农学通报, 2021, 37(28): 14-19.
[3]	王海燕, 陈新, 周新成, 沈旭, 孔华, 王文泉. Mechlppdk基因在木薯中的表达分析及RNA干扰载体构建和遗传转化[J]. 中国农学通报, 2021, 37(17): 19-25.
[4]	樊吴静,罗兴录,单忠英. 灌溉定额对膜下滴灌木薯产量及土壤理化性状的影响[J]. 中国农学通报, 2019, 35(28): 25-31.
[5]	吴秋妃①,王琴飞②,徐缓②,林立铭,李开绵②,张振文②. 不同生长期木薯叶片的品质比较分析[J]. 中国农学通报, 2019, 35(14): 54-61.
[6]	孟富宣,段元杰,杨玉皎,刘光华,刘海刚. 复盐胁迫对木薯幼苗光合特性及抗氧化酶活性的影响[J]. 中国农学通报, 2019, 35(1): 34-39.
[7]	李光义,余小兰,徐林,张红燕,邹雨坤,李勤奋. 木薯茎秆栽培基质氮磷钾养分变化规律研究[J]. 中国农学通报, 2019, 35(1): 40-44.
[8]	罗瑛,吴善坤,符乃方,欧文军. 七个木薯品种的氢氰酸含量研究[J]. 中国农学通报, 2018, 34(4): 38-42.
[9]	潘冉冉,秦于玲,乔景娟,赖杭桂,陈松笔. 14-3-3蛋白结构与功能预测及其在木薯成熟期的表达分析[J]. 中国农学通报, 2018, 34(36): 49-57.
[10]	栗欣如,尤飞. 基于ESDA 的广西木薯种植空间布局演变研究[J]. 中国农学通报, 2017, 33(5): 139-145.
[11]	余厚美,安飞飞,罗秀芹,陈松笔,欧文军. 淀粉磷酸化酶原核表达及单克隆抗体的制备[J]. 中国农学通报, 2017, 33(14): 28-32.
[12]	梁海波,魏云霞,宋恩亮,黄洁. 世界木薯生产对中国的启示[J]. 中国农学通报, 2016, 32(9): 94-99.
[13]	许成帅,李伯松,李克烈,王飞,郑丽,马旭东,覃新导. 烘干脱毒对木薯叶中氰化物和总黄酮含量的影响[J]. 中国农学通报, 2016, 32(5): 33-36.
[14]	郭运玲,孔华,左娇,黄启星,贾瑞宗,郭静远,周霞,郭安平. 木薯淀粉分支酶活性测定方法比较研究[J]. 中国农学通报, 2016, 32(33): 193-198.
[15]	付莉莉,沈培峰,谭德冠,孙雪飘,张家明. 高氰和低氰木薯生氰葡萄糖苷酶Linamarase基因的组织表达差异分析[J]. 中国农学通报, 2016, 32(11): 22-26.