烤烟黑胫病抗性评价及生理响应特性分析

doi:10.11924/j.issn.1000-6850.casb2023-0545

中国农学通报 ›› 2024, Vol. 40 ›› Issue (13): 102-108.doi: 10.11924/j.issn.1000-6850.casb2023-0545

烤烟黑胫病抗性评价及生理响应特性分析

代先强¹(), 刘念², 梁颖涛¹, 杨昌全¹, 邓可宣³, 陈少鹏¹()

¹ 中国烟草总公司重庆市公司，重庆 400020
² 四川省烟草公司宜宾市分公司，四川宜宾 645350
³ 西南大学农学与生物科技学院，重庆 400715

收稿日期:2023-07-27 修回日期:2024-01-19 出版日期:2024-04-28 发布日期:2024-04-28
通讯作者:
陈少鹏，男，1984年出生，河南新密人，农艺师，硕士，主要从事烟叶生产与管理研究。通信地址：400020 重庆市江北区五江路20号，Tel：023-67982915，E-mail： chenshaopeng3311@dingtalk.com。
作者简介:
代先强，男，1969年出生，重庆涪陵人，硕士，研究方向为农业推广。通信地址：400020 重庆市江北区五江路20号，Tel：023-67982918，E-mail：759788237@qq.com。
基金资助:
重庆市烟草专卖局科技项目“烤烟新品种抗青枯病性比较及配套防控技术研究”(C20221NY3433)

Resistance Analysis of Tobacco Black Shank Disease and Characteristic of Physiological Response

DAI Xianqiang¹(), LIU Nian², LIANG Yingtao¹, YANG Changquan¹, DENG Kexuan³, CHEN Shaopeng¹()

¹ Chongqing Tobacco Company of China Tobacco, Chongqing 400020
² Yibin Branch of Sichuan Tobacco Company, Yibin, Sichuan 645350
³ College of Agriculture and Biotechnology, Southwest University, Chongqing 400715

Received:2023-07-27 Revised:2024-01-19 Published:2024-04-28 Online:2024-04-28

摘要/Abstract

摘要：

本研究旨在揭示不同烤烟品种对烟草黑胫病的抗性机理。通过对抗病性的鉴定，从24个烤烟品种（系）中鉴定出4个高抗品种、8个中抗品种、8个中感品种和4个高感品种。对这些品种在烟草疫霉(Phytophthora parasitica var. nicotianae)侵染后，从中选取2个高抗品种和2个高感品种测定其抗氧化酶系活性、水杨酸含量以及抗病相关基因的表达情况。结果表明，在受到病原菌侵染时，高抗品种的抗氧化酶活性显著高于高感品种，暗示着更强的氧化应激防御能力。此外，高抗品种中的内源水杨酸合成以及抗病相关基因的诱导表达均显著高于高感品种，表明这些品种能够更有效地激活其防御系统以应对烟草黑胫病菌的侵染。本研究结果为理解烤烟对黑胫病的抗性机制提供了重要信息，并为烟草抗病育种提供了理论依据。

关键词: 烤烟, 黑胫病, 烟草疫霉, 抗病性, 抗氧化酶, 水杨酸, 基因表达

Abstract:

The study aimed to elucidate the resistance mechanisms of different tobacco varieties against tobacco black shank disease. The identification of resistance to tobacco black shank disease of 24 tobacco varieties (lines) was conducted, and four highly resistant varieties, eight moderately resistant varieties, eight moderately susceptible varieties, and four highly susceptible varieties were identified. Two highly resistant varieties and two highly susceptible varieties were selected to determine the activity of antioxidant enzyme system, salicylic acid content, and expression of disease-related genes after Phytophthora parasitica var. nicotianae infection. The results revealed that the antioxidant enzyme activity in highly resistant varieties was significantly higher than that in highly susceptible varieties after infection with P. nicotianae, suggesting that highly resistant varieties have stronger antioxidative capabilities. Additionally, the synthesis of endogenous salicylic acid in high-resistant varieties was significantly stronger than that in highly susceptible varieties after infection with P. nicotianae, and the expression of disease resistance genes was also significantly higher than that in highly susceptible varieties, indicating that resistant varieties can more efficiently trigger their own defense system to resist the invasion of P. nicotianae. The study offers insights into the mechanisms of tobacco against tobacco black shank disease and provides a theoretical foundation for breeding disease-resistant tobacco varieties.

Key words: flue-cured tobacco, black shank disease, disease resistance, Phytophthora nicotianae, antioxidant enzyme, salicylic acid, gene expression

代先强, 刘念, 梁颖涛, 杨昌全, 邓可宣, 陈少鹏. 烤烟黑胫病抗性评价及生理响应特性分析[J]. 中国农学通报, 2024, 40(13): 102-108.

DAI Xianqiang, LIU Nian, LIANG Yingtao, YANG Changquan, DENG Kexuan, CHEN Shaopeng. Resistance Analysis of Tobacco Black Shank Disease and Characteristic of Physiological Response[J]. Chinese Agricultural Science Bulletin, 2024, 40(13): 102-108.

图/表 9

参考文献 26

[1]	赵亚南, 黄大野, 杨丹, 等. 烟草黑胫病研究进展[J]. 湖北农业科学, 2022, 61(S1):25-28,66.
[2]	陈瑞泰, 朱贤朝, 王智发, 等. 全国16个主产烟省(区)烟草侵染性病害调研报告[J]. 中国烟草科学, 1997(4):3-9.
[3]	范志朋. 遵义市烟草黑胫病发病规律与化学防治研究[D]. 合肥: 安徽农业大学, 2020.
[4]	高文华, 李江美. 烟草黑胫病防治研究进展[J]. 热带农业科技, 2020, 43(1):50-54.
[5]	李永平, 肖炳光, 焦芳婵, 等. 烤烟新品种云烟97的选育及其特征特性[J]. 中国烟草科学, 2012, 33(4):28-31.
[6]	李永平, 王颖宽, 马文广, 等. 烤烟新品种云烟87的选育及特征特性[J]. 中国烟草科学, 2001(4):38-42.
[7]	张永春, 黄镇, 关国经, 等. 不同农业生态调控措施对烟草青枯病的影响[J]. 中国烟草科学, 2007(4):49-52.
[8]	赵显阳, 盘柳依, 陈明, 等. 茉莉酸甲酯对辣椒抗青枯病的诱导效应及抗氧化酶活性的影响[J]. 植物保护学报, 2018, 45(5):1103-1111.
[9]	GAO Y F, LIU J K, YANG F M, et al. The WRKY transcription factor WRKY8 promotes resistance to pathogen infection and mediates drought and salt stress tolerance in Solanum lycopersicum[J]. Physiologia plantarum, 2020,168:98-117.
[10]	韩宇婷, 陈晓晶, 徐忠山, 等. 不同燕麦品种抗氧化系统对秆锈病菌侵染的响应[J]. 北方农业学报, 2021, 49(4):98-104. doi: 10.12190/j.issn.2096-1197.2021.04.14
[11]	姜淑祯, 宋文静, 杨波, 等. 不同烤烟品种对青枯病胁迫的生理响应及抗性分析[J]. 中国烟草科学, 2022, 43(6):25-30.
[12]	QI P P, HUANG M L, HU X H, et al. A Ralstonia solanacearum effector targets TGA transcription factors to subvert salicylic acid signaling[J]. The plant cell, 2022,34:1666-1683.
[13]	TIBOR J, GABRIELLA S, MAGDA P. Salicylic acid signaling in plants[J]. International journal of molecular sciences, 2020, 21(7):2655. doi: 10.3390/ijms21072655 URL
[14]	DING P T, DING Y L. Stories of salicylic acid: A plant defense hormone[J]. Trends in plant science, 2020,25:549-565.
[15]	PIETERSE C M J, LEON-REYES A, VAN DER ENT S, et al. Networking by small-molecule hormones in plant immunity[J]. Nature chemical biology, 2009,5:308-316.
[16]	HILLMER R A, KENICHI T, GHANASYAM R, et al. The highly buffered Arabidopsis immune signaling network conceals the functions of its components[J]. PLoS genetics, 2017, 13(5):e1006639. doi: 10.1371/journal.pgen.1006639 URL
[17]	SATO M, TSUDA K, WANG L, et al. Network modeling reveals prevalent negative regulatory relationships between signaling sectors in Arabidopsis immune signaling[J]. PLoS pathogens, 2010, 6(7):e1001011. doi: 10.1371/journal.ppat.1001011 URL
[18]	LI B C, BASS W T, CORNELIUS P L. Resistance to tobacco black shank in Nicotiana species[J]. Crop science, 2006,46:554-560.
[19]	SAHU P K, JAYALAKSHMI K, TILGAM J, et al. ROS generated from biotic stress: Effects on plants and alleviation by endophytic microbes[J]. Frontiers in plant science, 2022,24:1042936.
[20]	KUNIAK E, KOPCZEWSKI T. The chloroplast reactive oxygen species-redox system in plant immunity and disease[J]. Frontiers in plant science, 2020,11:572686.
[21]	DAYMI C, ANGEL G C, ALEXANDER M. Reactive oxygen species, essential molecules, during plant-pathogen interactions[J]. Plant physiology and biochemistry, 2016,103:10-23.
[22]	LIANG M L, DONG L H, DENG Y Z. Circadian redox rhythm in plant-fungal pathogen interactions[J]. Antioxidants & redox signalling, 2022, 37(10/12):726-738.
[23]	APEL K, HIRT H. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction[J]. Annual review of plant biology, 2004,55:373-399.
[24]	ZHANG N, LV F, QIU F H, et al. Pathogenic fungi neutralize plant-derived ROS via Srpk1 deacetylation[J]. The EMBO journal, 2023, 42(9):e112634. doi: 10.15252/embj.2022112634 URL
[25]	郭明欣, 刘佳佳, 侯琳琳, 等. 植物体内活性氧的产生及清除机制研究进展[J]. 科技视界, 2021(8):104-106.
[26]	NADARAJAH K K. ROS homeostasis in abiotic stress tolerance in plants[J]. International journal of molecular sciences, 2020, 21(15): 5208.

基因ID	基因名称	引物名称	引物序列(5'-3')
NM_001326267.1	NtNPR1	NPR1-F	GAAACGCCTCTCGGAAACA
NM_001326267.1	NtNPR1	NPR1-R	TCCTCCGCTTATTGATATGC
NM_001325339.1	NtTGA2.1	TGA2.1-F	AAGTGGTACTATTGGAATGCCA
NM_001325339.1	NtTGA2.1	TGA2.1-R	GACTTCGATTGACATTAGCACC
XM_016633336.1/ X06361.1	NtPR1a/c	PR1a/c-F	AACCTTTGACCTGGGACGAC
XM_016633336.1/ X06361.1	NtPR1a/c	PR1a/c-R	GCACATCCAACACGAACCGA
XM_016643257.1	NtGAPDH	GAPDH-F	TGGGTGTCAACGAGAAGGAA
XM_016643257.1	NtGAPDH	GAPDH-R	TCTGGGTGGCAGTAAGGGA

基因ID	基因名称	引物名称	引物序列(5'-3')
NM_001326267.1	NtNPR1	NPR1-F	GAAACGCCTCTCGGAAACA
NM_001326267.1	NtNPR1	NPR1-R	TCCTCCGCTTATTGATATGC
NM_001325339.1	NtTGA2.1	TGA2.1-F	AAGTGGTACTATTGGAATGCCA
NM_001325339.1	NtTGA2.1	TGA2.1-R	GACTTCGATTGACATTAGCACC
XM_016633336.1/ X06361.1	NtPR1a/c	PR1a/c-F	AACCTTTGACCTGGGACGAC
XM_016633336.1/ X06361.1	NtPR1a/c	PR1a/c-R	GCACATCCAACACGAACCGA
XM_016643257.1	NtGAPDH	GAPDH-F	TGGGTGTCAACGAGAAGGAA
XM_016643257.1	NtGAPDH	GAPDH-R	TCTGGGTGGCAGTAAGGGA

品种	发病率/%	病情指数	鉴定类型	品种	发病率/%	病情指数	鉴定类型	品种	发病率/%	病情指数	鉴定类型
G27	61.3±3.7j	20.0±0.91m	R	湄育2号	81.3±4.0fgh	44.4±2.3j	MR	吉烟7号	87.1±1.1cdef	65.6±1.0ef	MS
G3	70.3±5.2i	22.2±0.7lm	R	MSY48	93.8±2.5abc	48.3±1.0i	MR	XD06	95.2±6.0ab	68.8±0.5de	MS
K326	82.1±6.1fgh	23.8±0.6l	R	云烟116	85.7±2.7defg	48.3±1.1i	MR	XD12	98.1±1.4ab	72.1±2.2cd	MS
革新三号	74.9±2.9hi	24.5±1.1l	R	XD08	75.0±2.9hi	49.0±0.5i	MR	许金2号	92.9±9.0abcd	73.4±1.9c	MS
湘烟7号	84.4±3.4efg	38.8±0.9k	MR	XD10	92.3±1.0bcd	51.3±0.6hi	MS	川烟1号	91.8±10.0bcde	78.9±0.9b	S
Oxford207	83.3±2.4fg	39.4±1.5k	MR	CF87	95.3±2.0ab	54.7±0.9gh	MS	红花大金元	100.0±0a	80.5±1.0ab	S
渝金香1号	92±1.4bcd	42.2±1.8jk	MR	贵烟36	93.3±2.5abcd	56.7±0.8g	MS	中烟207	97.1±1.8ab	80.5±1.7ab	S
贵烟14号	79.3±1.4gh	43.8±1.0j	MR	XD07	93.6±1.2abc	64.8±1.8f	MS	CF234	94.2±1.7abc	83.5±0.6a	S

品种	发病率/%	病情指数	鉴定类型	品种	发病率/%	病情指数	鉴定类型	品种	发病率/%	病情指数	鉴定类型
G27	61.3±3.7j	20.0±0.91m	R	湄育2号	81.3±4.0fgh	44.4±2.3j	MR	吉烟7号	87.1±1.1cdef	65.6±1.0ef	MS
G3	70.3±5.2i	22.2±0.7lm	R	MSY48	93.8±2.5abc	48.3±1.0i	MR	XD06	95.2±6.0ab	68.8±0.5de	MS
K326	82.1±6.1fgh	23.8±0.6l	R	云烟116	85.7±2.7defg	48.3±1.1i	MR	XD12	98.1±1.4ab	72.1±2.2cd	MS
革新三号	74.9±2.9hi	24.5±1.1l	R	XD08	75.0±2.9hi	49.0±0.5i	MR	许金2号	92.9±9.0abcd	73.4±1.9c	MS
湘烟7号	84.4±3.4efg	38.8±0.9k	MR	XD10	92.3±1.0bcd	51.3±0.6hi	MS	川烟1号	91.8±10.0bcde	78.9±0.9b	S
Oxford207	83.3±2.4fg	39.4±1.5k	MR	CF87	95.3±2.0ab	54.7±0.9gh	MS	红花大金元	100.0±0a	80.5±1.0ab	S
渝金香1号	92±1.4bcd	42.2±1.8jk	MR	贵烟36	93.3±2.5abcd	56.7±0.8g	MS	中烟207	97.1±1.8ab	80.5±1.7ab	S
贵烟14号	79.3±1.4gh	43.8±1.0j	MR	XD07	93.6±1.2abc	64.8±1.8f	MS	CF234	94.2±1.7abc	83.5±0.6a	S

烤烟黑胫病抗性评价及生理响应特性分析

Resistance Analysis of Tobacco Black Shank Disease and Characteristic of Physiological Response

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 26

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	孙计平, 王亚乐, 李丽华, 李雪君, 吕文钧, 孙焕. ‘YY021’烘烤特性及其杂种优势[J]. 中国农学通报, 2024, 40(9): 42-47.
[2]	张世杰, 杨波, 王宾刚, 刘云, 张喜峰, 王文丽, 王泽理, 李山. 有机肥对渭北旱塬烟区烤烟产质量的影响[J]. 中国农学通报, 2024, 40(8): 38-46.
[3]	薛彤彤, 谢萌蒙, 孙辉, 李冲伟. 外源添加苯甲酸对紫苏种子发芽和幼苗生长的影响[J]. 中国农学通报, 2024, 40(7): 72-77.
[4]	查宏波, 赵芳, 吕家锋, 龚林, 陈华, 高顺波, 王文栋, 郑兴琪, 孙浩宸, 张倍. 优化施肥调控对连作烤烟农艺性状和产质量的影响[J]. 中国农学通报, 2024, 40(6): 53-56.
[5]	张丽颖, 刘宇轩, 林云红, 熊茜, 徐炜, 段积有, 席家新, 周鹏, 杜宇, 白羽祥, 王戈, 王娜. ‘硃砂2号’和‘NC297’在楚雄烟区的适应性分析[J]. 中国农学通报, 2024, 40(4): 32-38.
[6]	关思慧, 柴亚倩, 杨元玲, 刘慧英, 郝庆, 刁明. 低温胁迫对石榴光合特性和抗氧化能力的影响[J]. 中国农学通报, 2024, 40(3): 66-75.
[7]	左雪倩, 谭静, 邓伟, 徐雨然, 吕莹, 张锦文, 余琴, 董维, 管俊娇, 李小林. 云南省杂交籼稻品种试验分析[J]. 中国农学通报, 2024, 40(3): 8-15.
[8]	张晓伟, 张轲, 倪明, 赵新梅, 张云芬, 李枝武, 杨树明. 垂直深旋耕下有机物料改良酸性土壤及烤烟产质量的调控效应[J]. 中国农学通报, 2024, 40(2): 52-60.
[9]	潘翠萍, 陶炼, 谢红江, 李华佳, 邓群仙, 王永清. 低温胁迫对枇杷生理特性及抗寒相关基因差异表达的影响[J]. 中国农学通报, 2024, 40(13): 64-69.
[10]	沈毅, 闫鼎, 蔡宪杰, 倪霞, 李德文, 未启立, 左伟标, 夏文凯, 桂龙凤, 赵声春, 刘振宁, 罗启鹏. 土壤调理剂与叶面钾肥配合施用对烟叶产质的影响[J]. 中国农学通报, 2024, 40(12): 33-37.
[11]	陈丽丽, 吕平, 吕少杰, 房明志, 李斌, 胡跃高, 薛绪掌, 康文艺. 不同波长LEDs光源对苦荞芽菜生长、生物活性物积累及抗氧化活性的影响[J]. 中国农学通报, 2024, 40(12): 45-52.
[12]	钟燕华, 景元书, 魏远, 李维韬, 吉梦宇. 保山烟区降水及其干旱风险性评价区划[J]. 中国农学通报, 2024, 40(11): 112-120.
[13]	张晓伟, 杨宗云, 倪明, 赵新梅, 王炽, 杨树明, 徐兴阳. 外源有机肥碳氮比对植烟红壤微环境及烟叶品质的影响[J]. 中国农学通报, 2024, 40(11): 49-54.
[14]	邓小强, 李思军, 侯建林, 朱林, 夏冰, 吴文信, 江智敏, 张仲文, 章程, 郑宏斌, 王卫民, 邓小华. 烤烟上部6片烟叶一次性采收成熟度的模糊综合评价[J]. 中国农学通报, 2024, 40(1): 118-127.
[15]	陈贵云, 王惠芳, 王铎, 施定国, 张智俊, 许东亚, 李云川, 孔德翠, 王娜, 王戈, 丁义, 白羽祥. 烟秆生物质炭基肥施用对烤烟产质量的影响[J]. 中国农学通报, 2024, 40(1): 12-19.