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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (30): 55-60.doi: 10.11924/j.issn.1000-6850.casb2023-0877

• 资源·环境·生态·土壤 • 上一篇    下一篇

不同有机废弃物载体对Bacillus megaterium有效性的影响

刘丙花1(), 吴瑜健2, 刘方春1, 丁延芹2, 刘幸红1, 马胜国3, 彭琳1, 于连家1, 马海林1()   

  1. 1 山东省林业科学研究院资源与环境研究所，济南 250014
    2 山东农业大学生命科学学院，山东泰安 271018
    3 山东省林业保护和发展服务中心，济南 250014
  • 收稿日期:2023-12-25 修回日期:2024-04-15 出版日期:2024-10-25 发布日期:2024-10-23
  • 通讯作者:
    马海林，男，1970年出生，山东菏泽人，研究员，硕士研究生，研究方向：土壤与植物营养。通信地址：250014 山东省济南市历下区文化东路42号 山东省林业科学研究院，Tel：0531-88557780，E-mail：。
  • 作者简介:

    刘丙花，女，1983年出生，山东临沂人，高级工程师，博士研究生，研究方向：土壤与植物营养。通信地址：250014 山东省济南市历下区文化东路42号 山东省林业科学研究院，E-mail：。

  • 基金资助:
    山东省重点研发计划（重大科技创新工程）项目“土壤结构改良微生物菌剂研发及生物强化关键技术研究”(2021CXGC010804)

Effect of Different Organic Wastes as Carrier on Effectiveness of Bacillus megaterium

LIU Binghua1(), WU Yujian2, LIU Fangchun1, DING Yanqin2, LIU Xinghong1, MA Shengguo3, PENG Lin1, YU Lianjia1, MA Hailin1()   

  1. 1 Institute of Resources and Environment, Shandong Academy of Forestry, Jinan 250014
    2 College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018
    3 Shandong Forestry Protection and Development Service Center, Jinan 250014
  • Received:2023-12-25 Revised:2024-04-15 Published:2024-10-25 Online:2024-10-23

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摘要：

为了给Bacillus megaterium选择理想的吸附载体。测定了草炭、金针菇渣、腐熟鸡粪、牛粪、羊粪的吸菌量及其载体菌剂的有效活菌数、胞外多糖（EPS）含量和发芽指数。草炭的吸菌量最高为1.64 mL/g，但随存放时间延长，草炭载体菌剂的有效活菌数和EPS含量急剧降低，且38 d内的发芽指数均小于80%，表现植物毒性。鸡粪和金针菇渣的吸菌量虽小于草炭，但随存放时间延长，鸡粪载体菌剂的有效活菌数和EPS含量逐渐增大，金针菇渣载体菌剂的EPS含量逐渐增加、有效活菌数降低幅度缓慢，且存放7 d后发芽指数均大于80%，表现植物无毒性。第38天时鸡粪和金针菇渣载体菌剂的有效活菌数分别为1.29×109 CFU/g和6.20×108 CFU/g，EPS含量分别为535.29 mg/kg和559.34 mg/kg，均显著高于其他载体菌剂。鸡粪和金针菇渣是B. megaterium较为理想的吸附载体。

关键词: 吸附载体, 吸附能力, 有机废弃物, 巨大芽孢杆菌, 有效活菌数, 发芽指数, 胞外多糖

Abstract:

In order to select the ideal carrier for Bacillus megaterium, grass charcoal, cow manure, sheep manure, chicken manure and enoki mushroom residue were used as carriers for B. megaterium to determine their effects on the adsorption capacity, effective viable bacteria number, exopolysaccharides (EPS) content and germination index of the bacterial solution. Charcoal had the highest bacterial liquid adsorption capacity which was 1.64 mL/g, but with the extension of storage time, the effective viable bacterial number and EPS content in the charcoal inoculum decreased sharply, and the germination index was less than 80% within 38 days, indicating the plant toxicity. Although the bacterial absorption capacity of chicken manure and enoki mushroom residue was smaller than that of grass charcoal, the effective viable bacterial number and EPS content in the chicken manure inoculum showed a trend of increasing. For the enoki mushroom residue inoculum, the EPS content increased gradually, and the effective viable bacteria number decreased slowly. The germination index of the two carrier inoculants was greater than 80% after 7 days of storage, indicating no plant toxicity. On the 38th day, the effective viable bacteria number of the chicken manure and enoki mushroom residue inoculants were 1.29×109 CFU/g and 6.20×108 CFU/g, respectively, the EPS content was 535.29 mg/kg and 559.34 mg/kg, respectively, which were significantly higher than other carrier inoculants. Chicken manure and enoki mushroom residue were the ideal carriers for B. megaterium.

Key words: carrier, adsorption capacity, organic waste, Bacillus megaterium, effective viable bacteria number, germinating index, exopolysaccharides