传染性法氏囊病毒安徽超强毒株的分离及分子鉴定

摘要/Abstract

摘要： 应用鸡胚绒毛尿囊膜(CAM)接种﹑琼脂扩散试验、电镜观察，从临床病鸡的法氏囊组织分别分离到3株传染性法氏囊病病毒（IBDV）CH03、JG04和QJ04株，对4周龄未免疫鸡的攻毒致死率分别为92%、83%、67%，对鸡胚的半数致死量（ELD50）分别为10-6.8/0.2ml、10-5.4/0.2ml、10-4.6/0.2ml；应用逆转录酶-聚合酶链式反应（RT-PCR）扩增的IBDV VP2基因高变区（vVP2）及其序列分析的结果表明，序列共有492个核苷酸，编码164个氨基酸。关键位点的氨基酸变化特征：第一亲水区P222A、疏水区的K249Q和S254G、N279D和T284A，与超强毒参考株UK661﹑HK46﹑OKYM和CH1-97极为相似，而与致弱株Cu-1）及变异毒株Var-E存在明显差异。研究结果表明，获得的3株IBDV分离株均属超强毒株。

关键词: 海南, 海南, 花卉业, 机遇, 挑战

Abstract: 3 strains of infectious bursal disease virus (IBDV) designated as CH03, JG04 and QJ04, were isolated and identified from bursa tissues of infected chickens of Anhui province by chicken embryo inoculation, AGP test and electronic microscopy. 4-week-old chickens, which were unvaccinated of IBDV, were inoculated with the tree isolates and experienced severe clinical disease with the mortalities of 92%, 83% and 67%, and the ELD50 of 3 isolates were 10-6.8/0.2ml, 10-5.4/0.2ml and 10-4.6/0.2ml, respectively. The VP2 hypervariable regions (vVP2) were amplified by RT-PCR (reverse transcriptase polymerase chain reaction) /Nested PCR and sequence analysis of the amplified products showed that the corresponding drifts of amino acids were found at five different epitopes: P222A located in the first hydrophilic region, K249Q, S254G, N279D and T284A located in the hydrophobic region, respectively. The results demonstrated that these 3 isolates were classified to be very virulent infectious bursal disease virus (vvIBDV) since they possess the epitope characteristics of vvIBDV but no that of attenuated or variant IBDV.

中图分类号:

S852.659.4

王桂军,吴忆春,魏建忠,李郁,何长生,韦平. 传染性法氏囊病毒安徽超强毒株的分离及分子鉴定[J]. 中国农学通报, 2006, 22(10): 17-17.

Wang Guijun, Wu Yichun, Wei Jianzhong, Li Yu, He Changsheng, Wei Ping. Isolation and Molecular Identification of Very Virulent Infectious Bursal Disease Virus[J]. Chinese Agricultural Science Bulletin, 2006, 22(10): 17-17.

参考文献

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