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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (8): 57-62.doi: 10.11924/j.issn.1000-6850.casb2024-0436

• 农学·农业基础科学 • 上一篇    下一篇

干旱-复水对烤烟‘YY021’苗期生理指标及干旱相关基因表达量的影响

李丽华1(), 薛冰洁2, 王亚乐2, 李雪君2, 孙焕2, 孙计平2()   

  1. 1 河南省烟草公司洛阳市公司，河南洛阳 471000
    2 河南省农业科学院烟草研究所，河南许昌 461000
  • 收稿日期:2024-07-03 修回日期:2024-10-15 出版日期:2025-03-15 发布日期:2025-03-14
  • 通讯作者:
    孙计平，女，1978年出生，河北玉田人，副研究员，博士，研究方向：烟草遗传育种。通信地址：461000 河南省许昌市永昌东路260号 河南省农业院烟草研究所，Tel：0374-3176781，E-mail：。
  • 作者简介:

    李丽华，女，1987年出生，河南濮阳人，农艺师，硕士，研究方向：烟草品种选育与栽培技术研究推广。通信地址：471000 河南省洛阳市洛龙区开元大道246号 洛阳市烟草公司，Tel：0379-65926068，E-mail：lilihua 。

  • 基金资助:
    河南省烟草公司洛阳市公司科技项目“洛阳烟区新品种选育及主栽品种改良”(2022410300200053)

Effects of Drought-rehydration on Physiological Indexes and Drought-related Gene Expression of ‘YY021’ Seedlings

LI Lihua1(), XUE Bingjie2, WANG Yale2, LI Xuejun2, SUN Huan2, SUN Jiping2()   

  1. 1 Luoyang Tobacco Company of Henan Province, Luoyang, Henan 471000
    2 Tobacco Research Institute, Henan Academy of Agricultural Sciences, Xuchang, Henan 461000
  • Received:2024-07-03 Revised:2024-10-15 Published:2025-03-15 Online:2025-03-14

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摘要： ‘YY021’是针对旱作烟区选育的烤烟杂交种，为了鉴定其耐旱性，以‘中烟100’为对照品种，采用漂浮育苗干旱-复水试验，通过调查萎蔫指数和含水量、观察活性氧变化、测定丙二醛和脯氨酸含量以及干旱相关基因表达量。结果表明，随着干旱时间延长，‘中烟100’和‘YY021’叶片含水量下降、萎蔫指数增加，活性氧增加，丙二醛含量增加，osmotin基因表达量先降低再增加；干旱7 d后，与‘中烟100’相比较，‘YY021’叶片含水量增加4.31%，萎蔫指数降低3.97%，丙二醛降低9.21%，脯氨酸含量增加84.71%；‘YY021’活性氧更低，osmotin基因表达量更高，ERD基因响应更迅速。研究发现，‘YY021’耐旱性优于‘中烟100’，干旱胁迫下，‘YY021’通过积累渗透调节物质脯氨酸以减轻活性氧对膜伤害，ERD和osmotin基因可能是‘YY021’耐旱候选基因。研究探索了‘YY021’耐旱机理，旨在为‘YY021’品种推广提供数据支持。

关键词: 烤烟, ‘YY021’, 耐旱性, 干旱-复水, 含水量, 丙二醛, 脯氨酸, 基因表达

Abstract:

‘YY021’ is a hybrid tobacco variety selected for dryland tobacco growing areas. In order to identify the drought tolerance of ‘YY021’, ‘Zhongyan 100’ was used as the control variety, and the drought-rehydration experiment of floating seedling was arranged to investigate wilting index and water content, observe the changes of active oxygen, and measure the content of malonaldehyde, proline and the expression of drought-related gene by qRT-PCR. The results showed that with the prolongation of drought time, the leaf water content of ‘Zhongyan 100’ and ‘YY021’ decreased and the wilting index increased. The content of malonaldehyde increased with the extension of drought time. The expression of osmotin gene decreased first and then increased from 0-7 d of drought. After 7 days of drought, compared with ‘Zhongyan 100’, the leaf moisture content of‘YY021’ increased by 4.31%, the wilting index of ‘YY021’ decreased by 3.97%, and the contents of malondialdehyde decreased by 9.21%, and the contents of proline increased by 84.71%. Compared with ‘Zhongyan 100’, ‘YY021’ had lower levels of reactive oxygen species and malondialdehyde, and the higher osmotin gene expression. The ERD gene responded more rapidly. The drought tolerance of ‘YY021’ was better than that of ‘Zhongyan 100’. Under drought stress, the damage of reactive oxygen species to membrane of ‘YY021’ was reduced by accumulating osmotic adjustment substance proline. ERD and osmotin genes may be candidate genes for drought tolerance of ‘YY021’.

Key words: flue-cured tobacco, ‘YY021’, drought tolerance, drought-rehydration, water content, MDA, proline, gene expression