勃氏甜龙竹笋采摘后低温保存下木质化动态研究

doi:10.11924/j.issn.1000-6850.casb2025-0053

中国农学通报 ›› 2025, Vol. 41 ›› Issue (24): 135-143.doi: 10.11924/j.issn.1000-6850.casb2025-0053

• 食品·营养·检测·安全 • 上一篇下一篇

勃氏甜龙竹笋采摘后低温保存下木质化动态研究

文裕卓(), 李璐稀, 许静云, 梁谢俊, 罗伟, 白天道()

广西大学林学院国家林草局中南速生材繁育重点实验室，南宁 530004

收稿日期:2025-01-23 修回日期:2025-05-29 出版日期:2025-08-25 发布日期:2025-09-05
通讯作者:
白天道，男，1983年出生，贵州遵义人，副教授，博士，主要从事林木遗传资源保育研究。通信地址：530004 广西壮族自治区南宁市西乡塘区大学东路100号，E-mail：btd@gxu.edu.cn。
作者简介:
文裕卓，男，2000年出生，广西南宁人，硕士，主要从事竹笋生长生理研究。通信地址：530004 广西壮族自治区南宁市西乡塘区大学东路100号，E-mail：wenyuzhuo0130@163.com。
基金资助:
广西重点研发计划“广西林下经济特色种质资源收集保存与生态高效栽培及利用研究”(2021AB20018)

Study on Lignification Dynamics of Dendrocalamus brandisii Shoots Under Low Temperature Preservation After Harvesting

WEN Yuzhuo(), LI Luxi, XU Jingyun, LIANG Xiejun, LUO Wei, BAI Tiandao()

College of Forestry, Guangxi University/Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast-Growing Timber in Central South China, Nanning 530004

Received:2025-01-23 Revised:2025-05-29 Published:2025-08-25 Online:2025-09-05

摘要/Abstract

摘要： 探究林下勃氏甜龙竹(Dendrocalamus brandisii)竹笋采摘后，不同低温保存方式对其木质化进程的延缓作用，以期为甜龙竹笋采摘后保鲜技术提供理论依据。以广西低丘林下勃氏甜龙竹笋为研究材料，分析其采摘后低温(4℃)真空贮藏以及低温密封（非真空）贮藏0~10 d内，木质素、粗纤维含量及木质素合成关键酶活性变化特征。结果显示：2种低温保存方式下1~10 d内，笋体木质素、粗纤维含量呈现不同程度增加趋势，真空处理可有效抑制43.21%的木质素合成量和28.61%的粗纤维合成量；真空处理下总酚含量始终保持持续下降趋势，而密封处理下总酚含量下降至第5天后，呈现一定程度回升；2种处理下，笋体的苯丙氨酸解氨酶(PAL)活性均呈现0~2 d内先下降后缓慢上升的趋势，真空处理下，第3天开始PAL活性极显著低于密封处理(P<0.001)。主成分及相关性分析表明，木质素、粗纤维、PAL活性与贮存天数呈正相关，与总酚呈负相关。试验表明，总酚、木质素、粗纤维含量和PAL活性是勃氏甜龙竹笋采摘后木质化进程中主要响应指标，4℃低温真空保存的物理保鲜方式，能有效延缓竹笋木质化进程和口感品质的快速下降。

关键词: 勃氏甜龙竹笋, 木质化, 低温真空保存, 酶活性

Abstract:

In order to provide a theoretical basis for the preservation technology of Dendrocalamus brandisii shoots after picking, the alleviating effects of different low temperature preservation methods on the lignification process of D. brandisii shoots after harvesting were investigated. In this study, the bamboo shoots of D. brandisii from low hilly forest in Guangxi were used as research materials to analyze the changes of lignin, crude fiber content and key enzyme activity of lignin synthesis within 0-10 days after low temperature (4℃) vacuum storage and low temperature sealed storage. The results showed that the contents of lignin and crude fiber in bamboo shoots increased in different degrees within 1-10 d under the two cryopreservation methods. Vacuum treatment could effectively inhibit the synthesis of 43.21% lignin and 28.61% crude fiber. The total phenol content under vacuum treatment always maintained a continuous downward trend, while the total phenolic content decreased under sealing treatment until the 5^th day and showed a certain degree of recovery. The phenylalanine ammonia lyase (PAL) activity of bamboo shoots under the two treatments showed a trend of decreasing first and then slowly increasing within 0-2 d, and the PAL activity under vacuum treatment was significantly lower than that under sealing treatment from the 3^rd day (P<0.001). Principal component analysis and correlation analysis showed that lignin, crude fiber and PAL activity were positively correlated with storage days, and negatively correlated with total phenols. The results showed that total phenol, lignin, crude fiber content and PAL activity were the main response indexes in the lignification process of D. brandisii shoots after harvesting. The physical preservation method of 4℃ low temperature vacuum preservation can effectively delay the lignification process and the rapid decline of taste quality of bamboo shoots.

Key words: Dendrocalamus brandisii shoots, lignification, cryopreservation, enzymatic activity

文裕卓, 李璐稀, 许静云, 梁谢俊, 罗伟, 白天道. 勃氏甜龙竹笋采摘后低温保存下木质化动态研究[J]. 中国农学通报, 2025, 41(24): 135-143.

WEN Yuzhuo, LI Luxi, XU Jingyun, LIANG Xiejun, LUO Wei, BAI Tiandao. Study on Lignification Dynamics of Dendrocalamus brandisii Shoots Under Low Temperature Preservation After Harvesting[J]. Chinese Agricultural Science Bulletin, 2025, 41(24): 135-143.

图/表 6

图1. 勃氏甜龙竹笋采摘后贮藏过程中外观形态变化

图2. 勃氏甜龙竹笋采摘后贮藏过程中木质化动态特征

图3. 木质化动态特征指标之间Pearson相关性分析图中*和***分别表示P<0.05和P<0.001

指标	Dim-1	Dim-2	Dim-3	Dim-4
CFC	0.9639	0.0812	0.1592	0.0926
LC	0.9055	0.2943	0.0986	-0.0403
TP	-0.5783	0.5626	0.1055	-0.5035
POD	0.0645	-0.0444	0.9500	-0.1788
PAL	0.2252	0.8635	-0.2269	0.0415
PPO	-0.5439	0.3880	0.3527	0.6284
特征值	2.4343	1.3078	1.1245	0.6923
累计贡献率/%	40.6	62.4	81.1	92.6

表1. 变量对主成分的载荷、特征值与贡献率

图4. 变量对主成分一和主成分二的贡献率

图5. 勃氏甜龙竹笋采摘后贮藏过程中木质化动态特征主成分分析

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勃氏甜龙竹笋采摘后低温保存下木质化动态研究

Study on Lignification Dynamics of Dendrocalamus brandisii Shoots Under Low Temperature Preservation After Harvesting

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