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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (10): 58-58.

所属专题： 生物技术

• 目次 • 上一篇    下一篇

拟康氏木霉产胞外多糖发酵培养基及培养条件的研究

郭 敏,陈靠山,   

  • 出版日期:2006-10-05 发布日期:2006-10-05

The study of liquid fermentive condition of Trichoderma pseudokoningii

Guo Min, Chen Kaoshan,   

  • Online:2006-10-05 Published:2006-10-05

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摘要： 以生物量和胞外多糖产量为指标，采用单因素和正交试验对摇瓶培养的拟康氏木霉（Trichoderma pseudokoning??）的液态发酵条件进行优化。优化后的拟康氏木霉发酵控制参数为：最佳培养基配方为麦麸30g/L，玉米粉30g/L，葡萄糖7.5g/L，KH2PO41g/L；发酵培养时间为6d；培养基的初始pH值为5.0～6.0；发酵温度为30±1℃。最佳培养条件下菌丝干重及胞外多糖的产量分别5.272g/L、0.622g/L。

关键词: 枯草芽孢杆菌纤溶酶(Bacillus subtilis fibrinolytic enzyme, 枯草芽孢杆菌纤溶酶(Bacillus subtilis fibrinolytic enzyme, BSFE), 转基因烟草, 表面活性剂, 根系分泌, 蛋白稳定性, 化学调节

Abstract: The liquid fermentative condition of Trichoderma pseudokoning?? were studied through single factor and orthogonal design tests. The experimental results showed the optimized fermentative conditions are as follows: Culture medium formula is wheat bran 30g/L, corn meal 30g/L, sucrose 7.5g/L,KH2PO41g/L. The optimum fermentation period is 6d. The fermentation temperature and the initial pH-value of culture medium is 30±1℃ and 5.0~6.0 respectively. The mycelial dry weigh and the extracellular polysaccharides of Trichoderma pseudokoningii reached 5.272 g/L,0.622 g/L under the the optimized fermentative conditions.

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