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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (10): 62-62.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

番茄果实特异性启动子2A11的基因克隆及功能研究

林炳英,,李 梅,林德钦,金志强   

  • 出版日期:2006-10-05 发布日期:2006-10-05

Cloning and Functional Analysis on Promoter of Tomato Fruit-specific 2A11 from Lycopersicon esculentum

Lin Bingying,, Li mei, Lin Deqin, Jin Zhiqiang   

  • Online:2006-10-05 Published:2006-10-05

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摘要： 采用巢式PCR技术从番茄基因组DNA克隆到长度1.3kp的果实特异性2A11启动子基因。序列分析表明，克隆到的基因序列2A11启动子转录起始位点上游的621bp处缺失了已报道的番茄2A11启动子基因（GenBank ID M87659，1993）序列中的“tatattgttaacttcttgttgaattaaagcaat”片段，其同源性为61%，登入GenBank，ID号为DQ453963；构建植物表达载体pCAMBIA2A11，用农杆菌介导侵染番茄果实，GUS基因瞬间表达结果表明，该2A11启动子基因具有驱动GUS基因在番茄果实中特异性表达的功能。研究结果表明成功地获得2A11果实特异性启动子基因，为下一步转基因番茄口服疫苗的研制奠定了一定的基础。

关键词: 硫肥, 硫肥, 烤烟, 产量, 品质

Abstract: The promoter of tomato fruit-specific 2A11 gene in tomato was amplified by nested-primers PCR technology. DNA sequence analysis and homology comparison indicated that the result was to be proved to absent the segment of “tatattgttaacttcttgttgaattaaagcaat”from 621bp upstream transcription start site of 2A11 promoter gene and to share 61% homology with 2A11 promoter (GenBank ID M87659，1993). DNA sequence had been submitted to GenBank, and its GenBank submission number was DQ453963. Binary vector was constructed through fusing 1.3kb 2A11 promoter gene with the GUS gene. Transient GUS expressions were observed in tomato fruits transferred by Agrobacterium tumefaciens EHA105. The result of GUS transient expression in tomato fruit indicated that 2A11 promoter had the function of driving the GUS gene fruit-specific expression in tomato fruit. Tomato fruit-specific 2A11 promoter had been successfully cloned in this paper, thus making possible the subsequent research in oral vaccine of transgenic tomato.

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