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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (4): 156-163.doi: 10.11924/j.issn.1000-6850.casb2025-0159

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

踩踏对深圳市公园绿地土壤微生物多样性的影响

刘贵迎1(), 彭李亚2, 王磊1()   

  1. 1 华南农业大学植物保护学院， 广州 510642
    2 深圳市农产品质量安全检验检测中心， 广东深圳 518101
  • 收稿日期:2025-02-26 修回日期:2025-07-16 出版日期:2026-02-27 发布日期:2026-02-27
  • 通讯作者:
    王磊，男，1986年出生，安徽界首人，副教授，博士，研究方向：昆虫生态学、害虫防治和入侵生物学等。通信地址：510642 广东广州天河区五山街道五山路483号 华南农业大学植物保护学院，E-mail：。
  • 作者简介:

    刘贵迎，男，1997年出生，山东临沂人，硕士研究生，研究方向：昆虫生态学和微生物生态学。通信地址：510642 广东广州天河区五山街道五山路483号 华南农业大学植物保护学院，E-mail：。

  • 基金资助:
    广东特支计划“科技创新领军人才项目”(2023TX07A017)

Impact of Recreational Trampling on Soil Microbial Community Structure in Urban Green Spaces: A Case Study of Shenzhen Parks

LIU Guiying1(), PENG Liya2, WANG Lei1()   

  1. 1 College of Plant Protection, South China Agricultural University, Guangzhou 510642
    2 Shenzhen Academy of Inspection and Testing for Agro-product Quality Safety, Shenzhen, Guangdong 518101
  • Received:2025-02-26 Revised:2025-07-16 Published:2026-02-27 Online:2026-02-27

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摘要：

城市绿地土壤微生物多样性作为生态系统健康性的重要指标备受关注。本研究以深圳市凤凰山人才林公园草坪区为研究对象，系统评估人为踩踏对土壤微生物群落的生态影响。通过高通量测序技术，系统分析踩踏扰动前后土壤微生物多样性、物种组成和菌群功能的变化特征，揭示踩踏对土壤微生物的负面影响机制。结果表明，踩踏可显著降低土壤微生物多样性，其中真菌物种丰富度下降23%，细菌Shannon多样性指数下降5%。主坐标分析(PCoA)和非度量多维尺度分析(NMDS)表明，踩踏显著改变草坪土壤微生物菌群结构，导致真菌和细菌操作分类单元(OTUs)数量减少。物种组成分析发现，拟酸杆菌门的相对丰度在踩踏后显著增加。功能分析显示，植物病原真菌的相对丰度在踩踏后显著增加，表明土壤健康风险增加。本研究量化了踩踏对城市绿地土壤微生物群落的负面影响，可为公园绿地的可持续管理及土壤修复提供科学依据。

关键词: 公园绿地, 踩踏, 土壤微生物多样性, 物种组成, 功能预测

Abstract:

The increasing significance of urban green spaces has drawn attention to soil microbial diversity within park green spaces as a crucial indicator of ecosystem health. In this study, the lawn area of Fenghuangshan Talent Forest Park in Shenzhen City was taken as the research object, and the ecological impact of human trampling on soil microbial community was systematically evaluated for the first time. By integrating high-throughput sequencing technology, a systematic analysis was conducted on the alterations in soil microbial diversity, species composition, and community functions before and after trampling disturbance. This methodology effectively uncovers the negative impact mechanisms of trampling on soil microbes. The results showed that there were significant decreases in the species richness index of soil fungi (23%) and the Shannon species diversity index of bacteria (5%) after trampling. PcoA and NMDS analyses indicated that there were significant differences in the Beta diversity of soil microorganisms after trampling, suggesting that trampling substantially altered the soil microbial community structure of the lawn. In terms of microbial species composition, the operational taxonomic units (OTUs) sequence numbers for both fungi and bacteria decreased after trampling, with a notable increase in the relative abundance of Acidobacteriota. The FUNGuild fungal functional classification indicated that the relative abundance of plant pathogenic fungi significantly increased after trampling. This study quantified the negative effects of trampling on soil microbial communities in urban green space, and provided a scientific basis for sustainable management and soil remediation of park green space.

Key words: park green spaces, trampling, soil microbial diversity, species composition, functional prediction