甜菜轮作田中3种鞘氨醇单胞菌的分离及最适促生浓度筛选

doi:10.11924/j.issn.1000-6850.casb2026-0222

中国农学通报 ›› 2026, Vol. 42 ›› Issue (12): 38-47.doi: 10.11924/j.issn.1000-6850.casb2026-0222

甜菜轮作田中3种鞘氨醇单胞菌的分离及最适促生浓度筛选

程迦南(), 王增澔, 高佑凯, 傅奕豪, 金成宇, 孙艳春()

黑龙江大学现代农业与生态环境学院，哈尔滨 150080

收稿日期:2026-03-23 修回日期:2026-04-14 出版日期:2026-06-25 发布日期:2026-06-23
通讯作者:
孙艳春，女，1987年出生，河南周口人，讲师，博士，研究方向：作物学、土壤微生物。通信地址：150080 黑龙江哈尔滨南岗区学府路74号黑龙江大学现代农业与生态环境学院，Tel：0451-86609487，E-mail：2017053@hlju.edu.cn。
作者简介:
程迦南，男，2002年出生，黑龙江人，硕士在读，研究方向：土壤微生物。通信地址：150080 黑龙江哈尔滨南岗区学府路74号黑龙江大学现代农业与生态环境学院，Tel：0451-86609487，E-mail：18814505132@163.com。
基金资助:
黑龙江省博士后面上项目“连作和轮作对甜菜土壤微生物群落结构及功能的影响”(LBH-Z23255)

Isolation of Three Sphingomonas Strains from Sugar Beet Rotation Soil and Screening of Their Optimal Growth-Promoting Concentrations

CHENG Jianan(), WANG Zenghao, GAO Youkai, FU Yihao, JIN Chengyu, SUN Yanchun()

College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080

Received:2026-03-23 Revised:2026-04-14 Published:2026-06-25 Online:2026-06-23

摘要/Abstract

摘要：

甜菜连作障碍已成为制约黑龙江甜菜产业高质量发展的突出问题，挖掘轮作体系有益微生物资源是实现绿色防控的关键途径。本研究旨在从黑龙江甜菜轮作土壤中筛选具促生潜力的鞘氨醇单胞菌、明确其最佳施用浓度，为甜菜连作障碍缓解提供菌种资源与理论依据。研究采用革兰氏染色、16S rDNA测序及PCR鉴定菌株，测定溶磷、固氮、解钾及产IAA能力，并通过盆栽试验确定最佳促生浓度。结果表明：分离获得3株鞘氨醇单胞菌，分别鉴定为W2 (Sphingobium abikonense)、W9 (Sphingomonas panni)、W13 (Sphingomonas sp.)。3株菌均产IAA，产量W13最强(58.21 mg/L)；仅W13初步表现出固氮能力，3株菌均无溶磷和解钾活性。盆栽试验表明，3株菌对甜菜幼苗均有显著促生效果，试验确定最佳促生浓度为W2：2.8×10⁹ CFU/mL，W9：4.2×10⁹ CFU/mL，W13：1.0×10⁸ CFU/mL。综上，本研究筛选的3株鞘氨醇单胞菌具有促生特性，可作为甜菜专用微生物菌剂候选菌株。未来可开展菌株复合配施、田间防效验证与促生分子机制研究，为甜菜连作障碍绿色防控提供新技术支撑。

关键词: 甜菜, 轮作, 连作障碍, 鞘氨醇单胞菌, 植物促生菌, IAA, 促生特性, 盆栽试验, 微生物菌剂

Abstract:

The continuous cropping obstacle of sugar beet has become a prominent problem restricting the high-quality development of sugar beet industry in Heilongjiang Province. Excavating the beneficial microbial resources of rotation system is the key way to realize green prevention and control. This study aimed to screen Sphingomonas strains with plant growth-promoting potential from sugar beet rotation soils in Heilongjiang Province, in order to alleviate continuous cropping obstacles. Bacterial strains were identified based on Gram staining, 16S rDNA sequencing, and PCR amplification. Their capacities for phosphate solubilization, nitrogen fixation, potassium solubilization, and IAA production were evaluated. Pot experiments were conducted to determine the optimal growth-promoting inoculation concentrations. Three Sphingomonas strains were successfully obtained, namely W2 (Sphingobium abikonense), W9 (Sphingomonas panni), and W13 (Sphingomonas sp.). All three strains were capable of synthesizing IAA, with strain W13 exhibiting the highest yield (58.21 mg/L). Only strain W13 displayed preliminary evidence of nitrogen fixation potential, whereas none of the three strains demonstrated phosphate- or potassium-solubilizing activity. Pot experiments revealed that the optimal growth-promoting concentrations were 2.8×10⁹ CFU/mL for W2, 4.2×10⁹ CFU/mL for W9, and 1.0×10⁸ CFU/mL for W13. In summary, the three isolated sphingomonas strains possess plant growth-promoting characteristics and can be used as candidate strains for special microbial agents for sugar beet. In the future, compound application of strains, field control effect verification and growth-promoting molecular mechanism research can be carried out to provide new technical support for green prevention and control of sugar beet continuous cropping obstacles.

Key words: sugar beet, crop rotation, continuous cropping, Sphingomonas, plant growth-promoting bacteria, IAA, growth-promoting characteristics, pot experiment, microbial agent

中图分类号:

S154.39

程迦南, 王增澔, 高佑凯, 傅奕豪, 金成宇, 孙艳春. 甜菜轮作田中3种鞘氨醇单胞菌的分离及最适促生浓度筛选[J]. 中国农学通报, 2026, 42(12): 38-47.

CHENG Jianan, WANG Zenghao, GAO Youkai, FU Yihao, JIN Chengyu, SUN Yanchun. Isolation of Three Sphingomonas Strains from Sugar Beet Rotation Soil and Screening of Their Optimal Growth-Promoting Concentrations[J]. Chinese Agricultural Science Bulletin, 2026, 42(12): 38-47.

图/表 11

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Grope	CK	W2-0.2	W2-0.4	W2-0.6	W2-0.8	W2-1.0
Height(cm)	9.81±0.66c	11.35±0.66b	11.17±0.45b	13.83±0.87a	13.11±0.73a	13.20±0.69a
SD (cm)	2.93±0.28b	3.27±0.34a	3.24±0.24a	3.54±0.36a	3.53±0.38a	3.32±0.40a
DW (g)	0.11±0.01c	0.23±0.03b	0.19±0.03b	0.36±0.03a	0.33±0.04a	0.35±0.06a
RDW (g)	0.03±0.01d	0.07±0.01c	0.08±0.01c	0.14±0.03a	0.11±0.03b	0.09±0.02bc

Grope	CK	W2-0.2	W2-0.4	W2-0.6	W2-0.8	W2-1.0
Height(cm)	9.81±0.66c	11.35±0.66b	11.17±0.45b	13.83±0.87a	13.11±0.73a	13.20±0.69a
SD (cm)	2.93±0.28b	3.27±0.34a	3.24±0.24a	3.54±0.36a	3.53±0.38a	3.32±0.40a
DW (g)	0.11±0.01c	0.23±0.03b	0.19±0.03b	0.36±0.03a	0.33±0.04a	0.35±0.06a
RDW (g)	0.03±0.01d	0.07±0.01c	0.08±0.01c	0.14±0.03a	0.11±0.03b	0.09±0.02bc

Grope	CK	W9-0.2	W9-0.4	W9-0.6	W9-0.8	W9-1.0
Height(cm)	9.81±0.66c	11.39±0.65b	12.46±0.74a	12.80±0.87a	12.77±0.45a	12.53±0.58a
SD (cm)	2.93±0.28c	3.19±0.35bc	3.21±0.26bc	3.36±0.40ab	3.49±0.43ab	3.64±0.26a
DW (g)	0.11±0.01c	0.17±0.03bc	0.27±0.03b	0.39±0.11a	0.32±0.05ab	0.32±0.07ab
RDW (g)	0.03±0.01d	0.06±0.01c	0.09±0.01b	0.07±0.01bc	0.12±0.03a	0.07±0.01bc

Grope	CK	W9-0.2	W9-0.4	W9-0.6	W9-0.8	W9-1.0
Height(cm)	9.81±0.66c	11.39±0.65b	12.46±0.74a	12.80±0.87a	12.77±0.45a	12.53±0.58a
SD (cm)	2.93±0.28c	3.19±0.35bc	3.21±0.26bc	3.36±0.40ab	3.49±0.43ab	3.64±0.26a
DW (g)	0.11±0.01c	0.17±0.03bc	0.27±0.03b	0.39±0.11a	0.32±0.05ab	0.32±0.07ab
RDW (g)	0.03±0.01d	0.06±0.01c	0.09±0.01b	0.07±0.01bc	0.12±0.03a	0.07±0.01bc

Grope	CK	W13-0.2	W13-0.4	W13-0.6	W13-0.8	W13-1.0
Height(cm)	9.81±0.66d	14.39±0.36a	13.28±0.67b	12.88±0.66b	12.00±0.62c	13.11±0.85b
SD (cm)	2.93±0.28c	4.11±0.65a	3.54±0.38b	3.46±0.41b	3.43±0.43b	3.81±0.48ab
DW (g)	0.11±0.01d	0.48±0.03a	0.32±0.03b	0.25±0.04c	0.33±0.04b	0.35±0.08b
RDW (g)	0.03±0.01d	0.15±0.02a	0.11±0.01bc	0.08±0.01c	0.13±0.03ab	0.15±0.04a

甜菜轮作田中3种鞘氨醇单胞菌的分离及最适促生浓度筛选

Isolation of Three Sphingomonas Strains from Sugar Beet Rotation Soil and Screening of Their Optimal Growth-Promoting Concentrations

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菌株编号	拉丁文名	相似度/%
W2	Sphingobium abikonense	99
W9	Sphingomonaspanni	100
W13	Sphingomonas sp.	99

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