抑食肼对甜菜夜蛾3龄及4龄幼虫的室内毒力测定及杀灭效果研究

doi:10.11924/j.issn.1000-6850.casb2025-0618

中国农学通报 ›› 2026, Vol. 42 ›› Issue (12): 199-203.doi: 10.11924/j.issn.1000-6850.casb2025-0618

抑食肼对甜菜夜蛾3龄及4龄幼虫的室内毒力测定及杀灭效果研究

张冰¹(), 刘洋¹, 李忠蔚¹, 闫光兰¹, 曹会刚², 李丹丹¹()

¹ 南阳师范学院/河南省昆虫生物学重点实验室/河南省昆虫生物学国际联合实验室，河南南阳 473061
² 河南喜夫农生物科技有限公司，河南开封 475515

收稿日期:2025-07-22 修回日期:2026-01-12 出版日期:2026-06-25 发布日期:2026-06-23
通讯作者:
李丹丹，女，1982年出生，河南南阳人，教授，主要从事农业昆虫与害虫防治研究。通信地址：473061 河南省南阳市卧龙路1638号南阳师范学院，E-mail：lidannytc@126.com。
作者简介:
张冰，女，2003年出生，河南南阳人，硕士研究生，研究方向：农业昆虫与害虫防治。通信地址：473061 河南省南阳市卧龙路1638号南阳师范学院，E-mail：964034798@qq.com。
基金资助:
河南省重点研发专项“农作物病虫草害新型纳米农药研制与农药减量增效技术集成示范研究”(251111113200); “基于核酸类新农药的农作物病虫害绿色防控关键技术研发与集成示范”(231111111000)

Study on Toxicity and Lethal Effects of 1,2-Dibenzoyl-1-tert-butylhydrazine on 3^rd and 4^th Instar Larvae of Spodoptera exigua

ZHANG Bing¹(), LIU Yang¹, LI Zhongwei¹, YAN Guanglan¹, CAO Huigang², LI Dandan¹()

¹ Nanyang Normal University/Henan Key Laboratory of Insect Biology/The International Joint Laboratory of Insect Biology in Henan Province, Nanyang, Henan 473061
² Xifu Nong Biotechnology Co., Ltd of Henan, Kaifeng, Henan 475515

Received:2025-07-22 Revised:2026-01-12 Published:2026-06-25 Online:2026-06-23

摘要/Abstract

摘要：

为了明确抑食肼对甜菜夜蛾的防治效果，加速其推广应用，本研究采用背板点滴法，设定250、500、1000、2000、4000 mg/L的抑食肼溶液，通过背板点滴法处理3龄和4龄甜菜夜蛾幼虫。结果发现，抑食肼能有效抑制甜菜夜蛾幼虫的生长发育和蜕皮进程，3龄甜菜夜蛾处理96 h后LC₅₀为1619.352 mg/L，4龄甜菜夜蛾处理96 h后LC₅₀为634.610 mg/L。同时，施用抑食肼后，甜菜夜蛾幼虫体色由正常褐色转变为淡青色或白色，且抑食肼浓度越高，效果越显著。甜菜夜蛾对抑食肼的剂量依赖效应不显著，综合考虑龄期及抑食肼浓度，250 mg/L抑食肼对3龄幼虫杀灭效果较其他浓度更优，500 mg/L抑食肼对4龄幼虫杀灭效果较其他浓度更优。该研究为抑食肼在甜菜夜蛾中的应用，以及与其他速效农药的复配使用奠定了基础。

关键词: 甜菜夜蛾, 抑食肼, 背板点滴法, 毒力测定, LC₅₀

Abstract:

To clarify the control effect of 1,2-Dibenzoyl-1-tert-butylhydrazine (RH-5849) against the Spodoptera exigua and to facilitate its application, this study conducted topical bioassays to evaluate the toxicity of RH-5849 on the 3^rd and 4^th instar larvae of S. exigua at concentrations of 250, 500, 1000, 2000 and 4000 mg/L. The results showed that RH-5849 significantly disrupted larval development and molting. The median lethal concentration (LC₅₀) was 1619.352 mg/L for 3^rd instar larvae after 96 h of exposure, and 634.610 mg/L for 4^thinstar larvae after 96 h. In addition, RH-5849 treatment induced morphological abnormalities, characterized by a progressive change in larval body color from natural brown to pale green or white. Moreover, the higher the concentration of RH-5849, the more significant the colors change. The dose-dependent effect of RH-5849 on S. exigua is not significant. Considering both larval stage and RH-5849 concentration, 250 mg/L RH-5849 demonstrates superior efficacy against 3rd-instar larvae compared to other concentrations, while 500 mg/L RH-5849 was more effective against 4th-instar larvae. These findings provide foundational data for the potential integration of RH-5849 into S. exigua management strategies, either as a standalone treatment or in combination with other insecticides.

Key words: Spodoptera exigua, 1,2-Dibenzoyl-1-tert-butylhydrazine (RH-5849), topical application, toxicity determination, median lethal concentration (LC₅₀)

中图分类号:

S435农作物病虫害及其防治

张冰, 刘洋, 李忠蔚, 闫光兰, 曹会刚, 李丹丹. 抑食肼对甜菜夜蛾3龄及4龄幼虫的室内毒力测定及杀灭效果研究[J]. 中国农学通报, 2026, 42(12): 199-203.

ZHANG Bing, LIU Yang, LI Zhongwei, YAN Guanglan, CAO Huigang, LI Dandan. Study on Toxicity and Lethal Effects of 1,2-Dibenzoyl-1-tert-butylhydrazine on 3^rd and 4^th Instar Larvae of Spodoptera exigua[J]. Chinese Agricultural Science Bulletin, 2026, 42(12): 199-203.

图/表 4

图1. 不同浓度抑食肼处理3龄甜菜夜蛾幼虫不同天数表型图标尺为1 cm，下同

图2. 不同浓度抑食肼处理3龄甜菜夜蛾幼虫不同天数的校正死亡率图内小写字母表示差异显著(P<0.05)，下同

图3. 不同浓度抑食肼处理4龄甜菜夜蛾幼虫不同天数表型图

图4. 不同浓度抑食肼处理4龄甜菜夜蛾幼虫不同天数的校正死亡率

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抑食肼对甜菜夜蛾3龄及4龄幼虫的室内毒力测定及杀灭效果研究

Study on Toxicity and Lethal Effects of 1,2-Dibenzoyl-1-tert-butylhydrazine on 3^rd and 4^th Instar Larvae of Spodoptera exigua

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抑食肼对甜菜夜蛾3龄及4龄幼虫的室内毒力测定及杀灭效果研究

Study on Toxicity and Lethal Effects of 1,2-Dibenzoyl-1-tert-butylhydrazine on 3rd and 4th Instar Larvae of Spodoptera exigua

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Study on Toxicity and Lethal Effects of 1,2-Dibenzoyl-1-tert-butylhydrazine on 3^rd and 4^th Instar Larvae of Spodoptera exigua