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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (18): 159-165.doi: 10.11924/j.issn.1000-6850.casb2026-0122

• 生物科学 • 上一篇    下一篇

一株野生桑黄菌的分离与培养条件优化

巫桂芬(), 李海露, 李文昭, 袁芳, 韦付好, 张伟, 李佳怡, 朱静瑜   

  1. 广西民族师范学院农业与生物学院， 广西崇左 532200
  • 收稿日期:2026-01-23 修回日期:2026-05-15 出版日期:2026-09-25 发布日期:2026-09-24
  • 作者简介:

    巫桂芬，女，1985年出生，副教授，博士，主要从事食药用菌栽培与育种。已发表学术论文31篇，获授权专利24项、软著4项、商标1个。通信地址：532200 广西崇左市江州区佛子路23号，广西民族师范学院，Tel：86-14795524029，E-mail：。

  • 基金资助:
    广西壮族自治区科学技术厅“中国-东盟边境地区珍稀野生桑黄资源挖掘及其亲缘关系分析”(2022GXNSFBA035524); 广西壮族自治区科学技术厅“‘八桂之光’访学研修计划（科技人才专项）项目”(桂科发〔2025〕35号); 崇左市科学技术局“黄金菇引种与节能减排栽培技术应用示范”(崇科20220618); 广西民族师范学院 “基于产学研融合模式下卜利村食用菌生产及其产业链发展研究”(2022FW072); 广西民族师范学院“边疆（崇左市）食用菌栽培技术推广与绿色产业链构建示范”(2025ZD016)

Optimization of Isolation and Cultivation Conditions for a Wild Sanghuangporus sp.

WU Guifen(), LI Hailu, LI Wenzhao, YUAN Fang, WEI Fuhao, ZHANG Wei, LI Jiayi, ZHU Jingyu   

  1. College of Agriculture and Biology, Guangxi Minzu Normal University, Chongzuo, Guangxi 532200
  • Received:2026-01-23 Revised:2026-05-15 Published:2026-09-25 Online:2026-09-24

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摘要：

本研究对野生桑黄关键培养参数进行了优化，旨在为桑黄的高效培养提供理论依据。以一株野生桑黄为材料，采用PDA培养基对其进行菌丝分离纯化，并探讨不同温度和不同营养条件对该桑黄菌丝生长的影响。结果表明：30℃显著优于20℃，更有利于桑黄菌丝的萌发与生长。碳源试验中，淀粉处理组的桑黄菌丝生长速度最快(3.5 mm/d)，且与其他处理组间存在显著性差异，葡萄糖处理组次之。氮源试验中，牛肉膏处理组的桑黄菌丝萌发时间最短，且菌丝粗壮，生长快；甘氨酸处理组的菌丝萌发时间长，生长速度慢。无机盐试验，氯化钙对桑黄菌丝的促进作用最为显著，菌丝日生长量为3.5 mm/d，氯化钠处理组次之。总体而言，该野生桑黄菌株的菌丝生长对温度和氮源表现出较强的敏感性，适宜的培养温度和氮源类型是促进其菌丝高效生长的关键因素。

关键词: 桑黄, 菌丝分离, 纯化培养, 条件优化, 生长速度, 生物学特性

Abstract:

This study optimized key cultivation parameters of a wild Sanghuangporus sp., aiming to provide a theoretical basis for efficient cultivation of Sanghuangporus sp.. Using a wild Sanghuangporus sp. as material, PDA medium was employed for mycelial isolation and purification culture, and the effects of different temperatures and nutritional conditions on the mycelial growth of this Sanghuangporus sp. were investigated. The results showed that 30℃ was more favorable than 20℃ for the germination and growth of Sanghuangporus sp. mycelia. In the carbon source experiment, the starch treatment group exhibited the fastest mycelial growth rate (3.5 mm/d), with significant differences compared to other groups, followed by the glucose treatment group. In the nitrogen source experiment, the beef extract treatment group had the shortest mycelial germination time, with robust and rapidly growing mycelia; the glycine treatment group showed prolonged germination time and slow growth. In the inorganic salt experiment, calcium chloride had the best promoting effect on Sanghuangporus sp. mycelia, with a daily mycelial growth of 3.5 mm/d, followed by the sodium chloride treatment group. The mycelial growth of this Sanghuangporus sp. strain is highly sensitive to temperature and nitrogen source, and appropriate culture temperature and nitrogen source type are key factors for promoting efficient mycelial growth.

Key words: Sanghuangporus sp., mycelial isolation, purification culture, condition optimization, growth rate, biological characteristics

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