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中国农学通报 ›› 2007, Vol. 23 ›› Issue (1): 355-355.

所属专题: 生物技术

• 目次 • 上一篇    下一篇

沙漠乔桑的组织培养与工厂化生产

石文山,唐旭日,李树丽   

  • 出版日期:2007-01-05 发布日期:2007-01-05

The Test Tube Seedling Growth of Root the Matrix Experimental Study to the Desert Tall Mulberry

Shi Whenshan, Tang XuRi, Li Shuli   

  • Online:2007-01-05 Published:2007-01-05

摘要: :选取沙漠乔桑不同的外植体,通过芽的诱导、试管苗的快速增殖、试管苗生根和驯化移栽筛选出适宜的工厂化育苗工艺流程。3—6月份选取沙漠乔桑待萌发冬芽、萌发侧芽,流水冲洗干净后,70%酒精浸10秒,然后用0.15%氯化汞灭菌5~10min,以不同组合的培养基对外植体进行诱导、快速繁殖、生根。结果表明:以MS+BA1.0mg/l(单位下同)+NAA0.1作诱导培养基,用MS+BA0.5+NAA0.05作增殖培养基进行快速繁殖,以1/2MS+NAA0.2作生根培养基,移栽到草炭:珍珠岩:蛭石=3:1:2的混合基质中。通过试验筛选出了一套快速繁殖体系,建立了适宜于沙漠乔桑工厂化生产的工艺流程。

关键词: 磷酸缓冲液, 磷酸缓冲液, 防护作用, 亚硫酸伤害, 幼苗

Abstract: Select by examinations the different and outside body of Increase of Joe's mulberry of the desert, the inducement, the tube seedling that passes the bud breeds quickly, the tube seedling takes root and tames to turn to move to plant to sieve a feat factory to turn to teach the seedling craft process. Summary 3—6 months select by examinations tall mulberry sprouts the hibernacula outside ,sprouts the lateral bud, after flowing water hurtle to clean, 70% alcohol immerses 10, then put out the germ with 0.15% chlorination mercury for 5~10 minutes, With the development medium of different combination outward the body of Increase carries on the inducement and breed, takes root quickly. The result enunciation: with the MS+ BA1.0mg/ l( unit next together)+ the NAA0.1 makes to induce to develop the medium , using the MS+ BA0.5+ the NAA0.05 makes to propagate to develop the medium to carry on to breed quickly, with the 1/2 MS+NAA0.2 makes to take root to develop the medium, moving to plant the turf: Pearl rock :Vermiculite=3:1:2 of hybrid quality of medium in , Passed to experiment to sieve a set of breed the system quickly ,build up feat in the desert of Joe mulberry factory turn the craft process of the production.

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