关键词: 离体叶片, 离体叶片, 扦插, 干烧心, 症状, 特征

Abstract: To solve the problems of oriental tobacco hybrid seeds production, some major factors relating cost and efficiency at crossing and pollination stage were studied. The results showed that pollination in different florescence had various influences on the percentage of seeding and fruiting. In the aspect of crossing success rate, it got higher burliness rate and lower wage cost for only one time of artificial pollination after the central flower of 60% plants florescence. In a method of three times of pollination with 3~5 days interval after 40% central flowers florescence, it got ceiling amount of seeds at, whereas efficiency of seeds production was lower than the former. Another was four times of pollination after 20% central flowers florescence leading to higher wage cost and poor efficiency of seeds production. Burliness rate varied not too much in same day at different time for hand pollination, but higher weight of thousand grain and more seeds per capsule were obtained, crossing at the time of 16:30~18:30, as well as seed output of average single plant was markedly increased. Therefore, the selection of starting the artificial pollination with two times after 40% central flowers florescence could not only raise the blossom utilization and acquire higher seed yield of average single plant, but wage cost was reasonable. The pollination time should be chosen at the time of 16:30~18:30 when not only would the seeds be replete, but the capsule seed quantity be abundant and high seed yield for definite area be prospective.