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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (4): 95-95.

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紫外辐照对产α-ALDC枯草芽孢杆菌的诱变效应

王 洲,杨超英,薛正莲   

  • 出版日期:2007-04-05 发布日期:2007-04-05

The Mutation Effect on a Strain Bacillus subtilis is Producing α-acetolactate decarboxylase by UV Irradiation

Wang Zhou, Yang Chaoying, Xue Zhenglian   

  • Online:2007-04-05 Published:2007-04-05

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摘要： 采用紫外诱变(波长260nm，功率15w)对一株产α-乙酰乳酸脱羧酶的枯草芽孢杆菌（Bacillus subtilis）BS059菌株进行诱变处理。结果表明，在紫外辐照时间为50s时，诱变效果较好。从正突变菌株中反复筛选，得到两株产酶量较高的菌株BS059-15和BS059-22，比原出发菌株的酶活分别提高了107.62%和162.25%。经过连续传代试验，证明其遗传性状稳定，为可遗传变异。

关键词: 白藜芦醇, 白藜芦醇, 次生代谢产物

Abstract: A study was made on Bacillus subtilis is strain BS059 with the production of α- acetolactate decarboxylase by the technology of UV irradiation (λ=260nm，W=15w). The result showed that optimal mutation effect was at the irradiation time of 50 seconds. After several screening tests, two mutant strain was named BS059-15 and BS059-22 whose enzyme activities was 107.62% and 162.25% higher than the original strain BS059 respectively. Then it was found that the yield of α-ALDC by the strain BS059-15 and BS059-22 were stable through successive transfer of culture.

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