盐胁迫对大白菜幼苗生理生化特性的影响

摘要/Abstract

摘要： 为了探讨大白菜耐盐机理、筛选耐盐性鉴定指标，建立苗期耐盐性鉴定方法，选用了６个具有不同耐盐性的大白菜材料，用Hoagland营养液水培方法，研究了不同浓度NaCl胁迫处理下大白菜盐害指数、脯氨酸含量、可溶性糖含量、游离氨基酸含量和丙二醛（MDA）含量的变化规律。结果发现，随着NaCl处理浓度的增加以及胁迫时间的延长，大白菜幼苗叶片中的MDA含量、脯氨酸含量、可溶性糖含量以及游离氨基酸含量均呈上升趋势。不同大白菜品种的盐害指数表现出不同程度的增加。盐害指数和MDA含量在不同品种间表现了明显差异，能较好地反映大白菜各品种苗期的耐盐性，可作为大白菜耐盐性的筛选指标。

关键词: 松果体细胞培养, 松果体细胞培养, MTT, 5-HT免疫组化, 山羊

Abstract: To study the salt-tolerant mechanism , select the salt-tolerant indicators and find a method for judging salt-tolerance of Chinese cabbage at seeding stage, Six cultivars with different salt tolerance were used in this study, and Hoagland solution culture method was applied to investigate the dynamic of several physical and biochemical indexes, such as the content of proline, soluble sugars, free Amino acid and MDA in the leaves of Chinese cabbage, under different NaCl-stress pressure. The results showed that, with the increase of salt concentration and the prolonging time of NaCl stress, the contents of proline, soluble sugar，free Amino acid and MDA increased gradually and the salt injury index increased differently among cultivars. The salt injury index and MDA content were distinguishable in various cultivars and could be used to select salt tolerant germplasm in Chinese cabbage at seeding stage．

中图分类号:

S642.2

薛莉,,张凤兰,于拴仓,朱月林,余阳俊,张德双,赵岫云,徐家炳. 盐胁迫对大白菜幼苗生理生化特性的影响[J]. 中国农学通报, 2007, 23(8): 287-287.

Xue Li,, Zhang Fenglan, Yu Shuancang, Zhu Yuelin, Yu Yangjun, Zhang Deshuang, Zhao Xiuyun, Xu Jiabing. Effects of NaCl Stress on Physiological and Biochemical Characteristics in Chinese cabbage Seedling[J]. Chinese Agricultural Science Bulletin, 2007, 23(8): 287-287.

参考文献

[1] 马春平,崔国文.10个紫花苜蓿品种耐盐性的比较研究[J].种子,2006,25(7):53-56.
[2] 李卫欣,陈贵林,赵利,等.NaCl胁迫下不同南瓜幼苗耐盐性研究[J].植物遗传资源学报,2006,7(2):192-196.
[3] 方先文,汤陵华,王艳平,等.耐盐水稻种质资源的筛选[J].植物遗传资源学报,2004,5(3):295-298.
[4] 杜中军,翟衡,罗新书,等.苹果砧木耐盐性鉴定及指标判定[J].果树学报,2002,19(1):4-7.
[5] NOITE K D,HAWSON A D,GAAGE D A.Proline accumulation and methylation to prolinbetaine in citrus:implications for genetic engineering of stress resistance[J].J Amer.Soc.Hort.Sic., 1997,122:8-13.
[6] STOREY R,WAIKER R R.Citrus and salinity[J].Sci Hort.,1999,78:39-81.
[7] 许祥明,叶和春,李国凤.植物抗盐机理的研究进展[J].应用与环境生物学报,2000(6):379-387.
[8] 章文华,刘友良.盐胁迫下钙对大麦和小麦离子吸收和分配及H+-ATP 酶活性的影响[J].植物学报,1993,35(6):135-140.
[9] Yeo A R,Flowers T J.The absence of an effect of the Na/Ca ratio on NaCl uptake by rice[J].New Phytol,1985,90:81-90.
[10] 张树清,张夫道,刘秀梅,等.NaCl对大白菜种子萌发和幼苗生长的影响[J].植物营养与肥料学报,2006,12(1):138-141.
[11] 张殿忠,汪沛洪,赵会贤.测定小麦叶片游离脯氨酸的方法[J].植物生理学通讯,1990,26(4):62-65.
[12] 邵雪玲,毛歆,郭一清.生物化学与分子生物学实验指导[M].武汉:武汉大学出版社,2003:159-160.
[13] 波钦诺克,X H.植物生物化学分析方法[M].荆家海,丁钟荣译.北京:科学出版社,1981:95-100.
[14] Heath P L,Parker L.Photoperoxidation in isolated chloropasts kinetics and stoichiometry of fatty acid peroxidation[J].Arch Biochem,1986,125:189-198.
[15] 杜中军,翟衡,罗新书,等.苹果砧木耐盐性鉴定及指标判定[J].果树学报,2002,19(1):4-7.
[16] 孙小芳,郑青松,刘友良.NaCl胁迫对棉花种子萌发和幼苗生长的伤害[J].植物资源与环境学报,2000,9(3):22-25.
[17] 沈禹颖,王锁民,陈亚明.盐胁迫对牧草种子萌发及其恢复的影响[J].草业学报,1999,8(3):54-60.
[18] 郭望模,傅亚萍,孙宗修,等.盐胁迫下不同水稻种质形态指标与耐盐性的相关分析[J].植物遗传资源学报,2003,4(3):245-251.
[19] 马雅琴,翁跃进.引进春小麦种质耐盐性的鉴定性评价[J].作物学报,2005,31(2):58-64.
[20] 汤章城,王育启,吴亚华等.不同抗旱品种高粱苗中脯氨酸积累的差异[J].植物生理学报,2002,12(2):154-162.
[21] Moftah A E,MichelB E.The effectof sodium choloride on solute potential and praline accumulation in soybean leaves[J]. PhysiolPlant,1987,83:238-240.
[22] 刘娥娥,宗会,郭振飞,等.干旱、盐和低温胁迫对水稻幼苗脯氨酸含量的影响[J].热带亚热带植物报,2000,8(3):235-238.
[23] 张俊莲,张国斌,王蒂.向日葵耐延性比较及耐盐生理指标选择[J].中国油料作物学报,2006,28(2):176-179.
[24] 孙志宾,齐兴云,张洪涛,等.NaCl对拟南芥(Arabidopsis thaliana)生长与渗透调节的影响[J].山东科学,2006,19(3).
[25] 毛桂莲,许兴,杨涓.NaCl和Na2CO3对枸杞的胁迫效应[J],干旱地区农业研究,2004,22(2):100-104.
[26] 郑国琦,许兴,邓西平,等.盐分和水分胁迫对枸杞幼苗渗透调节效应的研究[J].干旱地区农业研究,2002,20(2):56-59.
[27] 张云起,刘世琦,杨凤娟,等.耐盐西瓜砧木筛选和耐盐机理研究[J].西北农业学报,2003,12(4):105-108.
[28] 袁林,克热木.伊力,张利权.NaCl胁迫对阿月浑子实生苗活性氧代谢与细胞膜稳定性的影响[J].植物生态学报,2005,29(6)985-991.
[29] 肖雯,贾恢先,蒲陆梅.几种盐生植物抗盐生理指标的研究[[J].西北植物学报,2000, 20(5):818-825.