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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (9): 7-7.

• 生物技术科学 • 上一篇    下一篇

甘草离体培养物中总黄酮提取工艺的优化

葛淑俊,李广敏,马峙英,王文全,安金翠,孟义江   

  • 出版日期:2007-09-05 发布日期:2007-09-05

Extraction and Content Determination of Total Flavonoids of Cμltures of Glycyrrhiza uralensis Fisch.

Ge Shujun, Li Guangmin, Ma Zhiying, Wang Wenquan, An Jincui, Meng Yijiang   

  • Online:2007-09-05 Published:2007-09-05

摘要： 【研究目的】对甘草离体培养物中总黄酮提取和测定工艺进行优化,以建立在室内筛选高产细胞系和试管苗株系的方法；【方法】采用超声波提取技术，对提取液、浸泡时间、提取温度等进行优化；【结果】将样品以75%甲醇浸泡6h后在32℃下超声提取两次，比色法测定总黄酮含量，加样回收率为95.3%~101.4%，相对标准偏差为1.61%。测得甘草初代愈伤组织中总黄酮含量为0.0012%~0.05%，子叶愈伤组织调控后含量高达0.08%，试管苗中总黄酮含量为1.04%~1.31%，移栽后高达4.5%。【结论】此工艺准确度较高、稳定性好、简便可行，为优良细胞系的筛选和试管苗的早期鉴定奠定了技术基础。

关键词: 长期定位施肥, 长期定位施肥, 蔬菜保护地土壤, 磷素, 土壤剖面

Abstract: [objective] to optimize the extraction and determination method of the total flavonoids thus to screen the cell clones and plantlets with high contents, [method] the ultrasonic technique was used and several treatments were tried and screened, [result] the best condition was marinated 6 hours with 75% carbinol then ultrasonic extracted twice. And then the content was determined with colorimetry from cultures of Glycyrrhiza uralensis Fisch. The result showed the recovery rate was between 95.3% and 101.4% and the RSD was 1.61%. The total flavonoids of the initial calli was between 0.0012% and 0.05%, the improvement cotyledonary calli was up to 0.08%, the contents of the plantlets were between 1.04% and 1.31%, and after being transplanted was up to 4.5%. [conclusion] The rate of veracity of this method was high and this provided the determination of Glycyrrhiza cultures flavonoids products a feasible method, and those data offered a credible academic gist for authenticate of the experiment resource.

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