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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (8): 355-359.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

章丘大葱子叶再生体系建立及再生株倍性鉴定

穆春华,刘 霞,于元杰,王金辉   

  • 收稿日期:2008-05-04 修回日期:2008-06-16 出版日期:2008-08-08 发布日期:2008-08-08

Constitute of Regeneration System of Spring Onion Via Cotyledon and Judge of Tetraploid Plantlets

Mu Chunhua, Liu Xia, Yu Yuanjie, Wang Jinhui   

  • Received:2008-05-04 Revised:2008-06-16 Online:2008-08-08 Published:2008-08-08

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摘要： 以章丘大葱子叶为材料，研究了不同激素配比对愈伤组织诱导和继代、芽分化、芽点的伸长及根诱导的影响，并对获得的再生植株进行了鉴定。结果表明，诱导章丘大葱子叶产生愈伤组织、愈伤组织的继代培养、愈伤组织芽分化、芽点的伸长及诱导生根的最佳培养基及激素配比分别为：MS+2,4-D 2.0mg/L+KT 0.5mg/L、MS＋2,4-D 2.5mg/L＋KT 0.5mg/L、MS+2,4-D 0+KT 1.0mg/L、MS+KT 0.5mg/L和MS基本培养基；试管苗经驯化移栽后，共有22株成活，成活率为92%，对成活的再生植株进行形态学、叶片气孔及根尖染色体鉴定表明，共有3株发生变异，其中2株为外部形态上的变异，而另一株为染色体的加倍变异。

关键词: 兰州市, 兰州市, 畜牧业, 发展现状, 存在问题, 发展建议

Abstract: Zhangqiu spring onion was used as experimental materials. We studied the optimum medium and hormone combination of callus induction from cotyledons, subculture of callus, shoot regeneration from callus, shoot-extension induction, root induction and identified the chromosome number of the plantlet. The results showed that: MS+2,4-D 2.0mg/L+KT 0.5mg/L, MS＋2,4-D 2.5mg/L＋KT 0.5mg/L, MS+2,4-D 0+KT 1.0mg/L, MS+KT 0.5 mg/L and basal MS medium. One plantlet among twenty two was determined as a tetraploidy based on the plant morphology, observation of the leaf stoma and chromosome number of the root, other two plantlets represented morphological variation.

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