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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (10): 83-87.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

苎麻4-香豆酸辅酶A连接酶-1基因的克隆与分析

陈建荣,郭清泉   

  • 收稿日期:2008-08-15 修回日期:2008-08-20 出版日期:2008-10-05 发布日期:2008-10-05

Cloning and Analysis of the 4-coumarate-CoA Lignase Gene-1

CHEN Jian-Rong   

  • Received:2008-08-15 Revised:2008-08-20 Online:2008-10-05 Published:2008-10-05

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摘要： 以苎麻为材料，采用PCR方法，以简并引物从苎麻基因组DNA模板中，首次克隆了4-香豆酸辅酶A连接酶-1基因（4CL-1）的DNA部分序列，长度为983 bp；采用RT-PCR的方法，以特异引物，从苎麻茎RNA反转录的cDNA为模板中，首次克隆了苎麻4CL-1cDNA核心序列，长度为110 bp；经生物信息学方法分析，得知所获得的基因序列编码一段含37个氨基酸残基的多肽，该多肽与几个酰基合成酶和AMP结合酶的同源性较高，可以推论其编码了AMP形成与结合的保守区域。

关键词: 大豆, 大豆, 超高产, 潜力, 展望

Abstract: Boehmeria nivea (Linn.) Gaud. cultivar “Zhongzhu No1” has been used, 4CL-1 genomic DNA sequence has been cloned first time by PCR with degenerate primers, the length of the sequence is 983 bp; 4CL-1 cDNA sequence has been cloned first time by RT-PCR with special primers from ramie stem, the length of the sequence is 110 bp; Bioinformatics analysis shows that this sequence encode a polypeptide about 37 amino acid residue, this polypeptide is similar to acyl-CoA synthetases, and related to AMP forming and binding.