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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (2): 168-171.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

樱桃不同杂种优系的组织培养与快速繁殖

孙国利,闫国华,张开春,张晓明,周宇,于泽源   

  • 收稿日期:2008-10-16 修回日期:2008-11-17 出版日期:2009-01-20 发布日期:2009-01-20

In Vitro Culture and Micropropagation of Different Hybrids in Cherry Plants

  • Received:2008-10-16 Revised:2008-11-17 Online:2009-01-20 Published:2009-01-20

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摘要： 以樱桃的优良杂种优系F8、F10（Prunus.avium L.×Prunus . pseudocerasus L.）以及H8、H10（Prunus. cerasus L ×Prunus.pseudocerasus L.）为材料，MS和F14为基本培养基，通过添加不同浓度的6-苄基氨基腺嘌呤(6-BA)、 3-吲哚丁酸(IBA) 、赤霉素（GA3）进行杂种樱桃组织培养和快速繁殖技术的研究。结果表明：F14+6-BA （0.3～0.5）mg/L+GA30.1(单位下同)适合各个杂种优系初代培养诱导出植株，F14+6-BA （0.5～1.0）+IBA （0.1～0.3）+GA30.1能够很好的促进各个杂种优系分化不定芽，1/2MS+IBA0.5（或NAA 0.8）是各个杂种优系的最佳生根培养基。

关键词: 小麦, 小麦, 玉米, 限制因素, 增产, 对策

Abstract: In Vitro culture and micropropagation for different selections of cherry plant hybrid including F8 and F10 (P. avium L.× P. pseudocerasus L.), H8 and H10 (P. cerasus L × P. pseudocerasus L.) were studied in this paper. It was showed that by taking MS and F14 basic medium supplemented with different combinations of plant growth regulators such as 6-BA, IBA and GA3, the micropropagation technologies including preliminary culture, subculture and rooting culture were successfully established. Medium F14+6-BA (0.3～0.5)mg/L+GA30.1mg/L is suitable for preliminary culture of the tested selections, F14+6-BA (0.5-1.0) mg/L +IBA0.1 mg/L +GA30.1 mg/L is an optimal subculture medium for proliferation of the In Vitro shoots, satisfactory rooting response can be obtained on medium 1/2MS+IBA0.5 ( or NAA 0.8) mg/L.