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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (4): 21-23.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

MEF2a基因在肌肉组织中的表达研究

高智晟,,杨 龙,张冬杰,刘 娣,刘玉芬   

  • 收稿日期:2008-10-22 修回日期:2008-12-01 出版日期:2009-02-20 发布日期:2009-02-20

Research on Expression of MEF2 Gene in Muscle Tissue

  • Received:2008-10-22 Revised:2008-12-01 Online:2009-02-20 Published:2009-02-20

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摘要： 分别取90日龄、180日龄和270日龄大白猪心肌、背肌和腿肌，提取总RNA，设计并合成猪MEF2a引物，以β肌动蛋白作为内参，利用Real-time PCR方法检测不同日龄大白猪肌肉组织中MEF2a基因mRNA的表达。结果表明，不同日龄大白猪的心肌和背肌中，MEF2a基因mRNA的表达水平无显著差异，在腿肌中有显著差异（P<0.05）。从90日龄到270日龄，MEF2a基因在心肌、背肌和腿肌的表达水平均差异显著（P<0.05），腿肌最高，其次是背肌，心肌最低。据此推测，MEF2a基因在猪成体后的肌肉组织中，主要是促进Ⅰ型肌纤维的生成，而不再是介导细胞分化。

关键词: 茄子, 茄子, 光合速率日变化, 光合“午休”, 生理生态因子

Abstract: The expression levels of 90d, 180d and 270d Landrace cardiac muscle, dorsal muscle and leg muscle were determined by Real-time PCR.Total RNA was extracted from muscle. The MEF2a PCR primers was designed according to blast homologat the conserved regions.β-actin was used as loading controls.The results showed that the cardiac muscle and dorsal muscle mRNA levels were not significantly varied among different day, expect leg muscle (P<0.05). From 90d to 270d, MEF2a expressions level were significant varied among cardiac muscle, dorsal muscle and leg muscle (P<0.05), and the highest is leg muscle, the second is dorsal muscle, the lowest is cardiac muscle. It was deduced that the main function of MEF2a is to promote I myofibrillar formation and not to mediate cell differentiation during adult pig muscle