东南亚茄子种质资源ISSR遗传多样性分析

doi:10.11924/j.issn.1000-6850.2014-0488

摘要/Abstract

摘要： 为探讨东南亚和中国华南地区茄子育种资源的遗传差异，研究采用ISSR分子标记对来自泰国和马来西亚的10份茄子材料及中国和南美的8份材料进行遗传多样性分析。结果表明，17条ISSR引物共扩增出433条谱带，其中多态性条带390条，平均多态率为92%。17条引物在18份材料中的平均观测等位基因数、平均有效等位基因数、平均Nei’s基因多样度和平均香农信息指数分别为1.8971、1.4778、0.2850、0.4336，18份材料间的遗传相似系数GS在0.55~0.81之间。运用NTSYSpc2.10软件的UPGMA方法进行聚类分析，结果表明在遗传相似系数0.67时18份材料可分为2个类群，其中来自于东南亚的10份材料在第1类群，来自于南美的3份材料在第2类群。研究结果表明，东南亚茄子材料与中国和南美茄子材料间遗传多样性丰富，可为茄子种质资源的进一步研究和利用提供理论依据。

关键词: 内生菌, 内生菌, 苦楝, 多样性, 次生代谢产物

Abstract: To clarify genetic diversity and relationship among eggplant germplasm, 18 accessions of eggplant including 10 from Southeast Asia, 3 from South America and 5 from China were assessed with ISSR markers. The results showed that 433 loci were amplified by 17 ISSR primers, and 390 loci were polymorphic bands, the percentage of polymorphic loci was 92%. The average observed number of alleles, average effective number of alleles, average Nei’s gene diversity index, and average Shannon’s information index were 1.8971, 1.4778, 0.2850, and 0.4336, respectively. The genetic similarity among all accessions ranged from 0.55 to 0.81. The 18 accessions of eggplant were clustered into two groups by UPGMA when GSC was 0.67, 10 accessions from Southeast Asia were clustered in group I, 3 accessions from South America in group II. The result showed that there was rich genetic diversity in eggplant cultivars between those from Southeast Asia and those from China and South America, provided a theoretical basis for further research and breeding of eggplant.

李涛黎振兴罗少波李植良徐小万. 东南亚茄子种质资源ISSR遗传多样性分析[J]. 中国农学通报, 2014, 30(25): 104-110.

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