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中国农学通报 ›› 2009, Vol. 25 ›› Issue (10): 1-5.

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

乳酸粪肠球菌的分离鉴定与系统进化分析

杨杨 霞,陈 陆,常洪涛,刘红英,杨影影,符 峰,王川庆   

  • 收稿日期:2008-12-08 修回日期:2009-02-08 出版日期:2009-05-20 发布日期:2009-05-20

Isolation,Identification and Phylogenetic Analysis of a Strain Enterococcus Faecalis

Yang Xia, Chen Lu, Chang Hongtao, Liu Hongying, Yang Yingying, Fu Feng, Wang Chuanqing   

  • Received:2008-12-08 Revised:2009-02-08 Online:2009-05-20 Published:2009-05-20

摘要: 目的 分离鉴定一株疑似乳酸粪肠球菌;方法 通过对表型特性包括培养特性、形态学观察、生化试验等鉴定,同时结合16S rDNA系统进化分析;结果:该株革兰氏阳性菌能在10℃和45℃生长,接触酶阴性,发酵葡萄糖产酸不产气,可生长于6.5%NaCl和PH9.6的环境等,16S rDNA系统进化分析与粪肠球菌有最高同源性;结论 分离株为乳酸粪肠球菌。

关键词: 白肋烟, 白肋烟, 株系, 试验, 农艺性状, 经济性状, 品质性状

Abstract: Abstract: Objective Isolation and Identification of a doubtful enterococcus faecalis strain;Methods through Phenotypic Characteristics containing culture characteristics, Morphological observation, biochemistry experiment etc, combining with the phylogenetic analysis of 16S rDNA; Results The result indicated that this Gram-positive bacteria can grow at 10 ℃ and 45℃,6.5% sodium chloride or pH9.6 also.catalase- negative,can produce acid and no gas from glucose. though phylogenetic analysis of 16S rDNA,we can found that it has topmost homogeneity with E.faecalis;Conclusion These findings may indicated that the isolated strain should be E.faecalis.

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