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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (9): 148-151.

所属专题： 生物技术； 小麦； 农业气象

• 农学 农业基础科学 • 上一篇    下一篇

外源腐胺对干旱胁迫下小麦叶片渗透调节的影响

赵文才,李慧,赵会杰,韩燕来,谭金芳   

  • 收稿日期:2009-02-18 修回日期:2009-03-18 出版日期:2009-05-05 发布日期:2009-05-05

Effects of Exogenous Polyamine Osmotica Regulation in Wheat Seedling leaves under Drought Stress

  • Received:2009-02-18 Revised:2009-03-18 Online:2009-05-05 Published:2009-05-05

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摘要： 用15%聚乙二醇（PEG-6000）模拟干旱胁迫处理，研究外源腐胺对干旱胁迫下冬小麦幼苗叶片膜质过氧化和有机渗透调节物质的影响。结果表明：在干旱胁迫条件下，外源腐胺显著提高了冬小麦叶片可溶性糖（SS）、游离脯氨酸（Pro）、游离氨基酸（TFA）含量，分别为干旱胁迫处理的1.73倍，9.51倍，1.5倍；降低了冬小麦叶片丙二醛（MDA）含量和质膜透性（PMP），与干旱胁迫处理相比下降了82%和84.5%，显著降低了叶片膜质过氧化程度。这说明外源腐胺能改善冬小麦幼苗叶片干旱胁迫下的渗透调节能力，从而增强植株的耐旱性。

关键词: 水稻种子, 水稻种子, 生物技术, 叶色标记, 纯度鉴定

Abstract: An experiment was conducted to study the effect of exogenous Putrescine on membrane lipid peroxidation and soluble organic osmoticum in wheat seedling leaves under drought stress induced by polyethylene glycol (PEG-6000). The results showed that the soluble sugar contents, Free proline contents and free amino contents were markedly increased that the ones were 1.73 times, 9.51 times and 1.5 times than that of control, malondialdehyde（MDA）contents and membrane permeability（PMP）were significantly decreased, which were 82% and 84.5% lower than those of MDA and PMP in the control, the grade of membrane lipid peroxidation was significantly reduced. The above results indicate that exogenous Putrescine could improve osmotic adjustment ability of winter wheat seedling leaves under drought stress and increase drought tolerance of plant.