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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (9): 51-54.

所属专题： 水稻

• 生物技术科学 • 上一篇    下一篇

海绵共附生放线菌Streptomyces sp. A01059抗稻瘟病活性物质的分离研究

彭 杰,,吴晓鹏,张开山,黄惠琴,孙前光,鲍时翔   

  • 收稿日期:2009-03-03 修回日期:2009-03-18 出版日期:2009-05-05 发布日期:2009-05-05

Separation of Active Compounds Against Pyricularia oryzae from sponge-associated actinobacteria Streptomyces sp. A01059

  • Received:2009-03-03 Revised:2009-03-18 Online:2009-05-05 Published:2009-05-05

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894

摘要： 摘要：目的 对海绵共附生放线菌Streptomyces sp. A01059的发酵液进行研究，以获得有抗稻瘟病活性的化合物；方法 用乙酸乙酯对发酵液进行萃取，然后用活性追踪的方法对乙酸乙酯萃取物进行分析，最后使用制备液相获得相应的活性部位；结果 获得分子量为508和522的两个活性化合物；结论 本实验首次对放线菌Streptomyces sp. A01059的代谢产物进行分离研究，为稻瘟病的防治提供了新的途径。

关键词: 牡丹, 牡丹, 紫纹羽病, 病原菌, 生物学特性, 防治

Abstract: Abstract: OBJECTIVE The fermentation broth of marine sponge-associated actinobacteria Streptomyces sp. A01059 was studied for the valuable active compounds which against rice blast; METHOD The fermentation broth was extracted with ethyl acetate, then activity-guided method was used to analysis the extraction and the active compounds were obtained by preparative liquid chromatography; RESULTS Two active compounds were obtained and the molecular weight were 508 and 522, respectively; CONCLUSION The metabolites of Streptomyces sp. A01059 were studied in this paper for the first time systematically, and a new method was provided to control rice blast.