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中国农学通报 ›› 2009, Vol. 25 ›› Issue (9): 47-50.

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

pCAMBIA2300-betA-BADH双价基因植物表达载体的构建

咸 洋,,夏 阳,张金文,庞彩红,李 丽,毛秀红   

  • 收稿日期:2009-01-23 修回日期:2009-02-11 出版日期:2009-05-05 发布日期:2009-05-05

Construction of plant expression vector of pCAMBIA2300-betA-BADH

  • Received:2009-01-23 Revised:2009-02-11 Online:2009-05-05 Published:2009-05-05

摘要: 摘要:本实验以载体pCAMBIA2300-35s-OCS为基础通过分子克隆手段,将甜菜碱合成途径的两个关键酶基因,即编码胆碱脱氢酶(CDH)的betA基因和编码甜菜碱醛脱氧酶(BADH)的BADH基因及启动子和终止序列构建在同一植物表达载体上,得到pCAMBIA2300-betA-BADH双价植物表达载体。并将其导入根癌农杆菌GV3101和LBA4404,进一步用于双子叶植物的遗传转化。

关键词: 茶树, 茶树, 生长量, 拟合公式

Abstract: Abstract: The choline dehydrogenase(CDH)gene and the betaine aldehyde dehydrogenase(BADH)gene are the key genes of the glycine betaine’s synthesis pathway. They were cloned into the vector pCAMBIA2300-35s-OCS.They were tandem and have their respective promoters and terminators. The vector pCAMBIA2300-betA-BADH was transformed into the Agrobactrium tumefaciens GV3101, LBA4404 and will be used in the genetic transformation of the dicotyledons.