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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (9): 43-46.

所属专题： 生物技术； 油料作物； 农业生态

• 生物技术科学 • 上一篇    下一篇

大豆灰斑病1号生理小种抗性基因的SSR标记

陈立君,郭强,刘迎雪,李祥羽,赵远玲,单大朋,陈庆山   

  • 收稿日期:2009-01-09 修回日期:2009-02-13 出版日期:2009-05-05 发布日期:2009-05-05

SSR Markers for Resistance Gene to Cercospora sojina Hara Race 1

  • Received:2009-01-09 Revised:2009-02-13 Online:2009-05-05 Published:2009-05-05

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摘要： 针对中国大豆灰斑病1号生理小种，以抗所有生理小种的品系东农40566为母本，以感所有生理小种的品种东农410为父本配制杂交组合，杂交得到F2代后连续自交3代得到F5代群体。该群体经人工接种灰斑病1号生理小种后,利用BSA法对500个SSR标记进行筛选,其中3个标记Satt565、SOYGPATR和Satt396在抗、感池间表现出稳定的多态性,并且在F2代个体中表现出抗性与多态性协同分离的趋势。3个标记与抗性基因的连锁顺序为Satt565—SOYGPATR—Hrcs1—Satt396,它们与抗性基因的连锁距离分别为12.7cM、6.5cM、14.7cM。推测抗大豆灰斑病1号生理小种的基因可能位于C1连锁群上。

关键词: 紫茎泽兰, 紫茎泽兰, 环境条件, 种子萌发

Abstract: In aim to find the SSR markers linked to resistance gene against frogeye leaf spot in Chinese soybean, Bulked segregant analysis was applied to F5 populations derived from cross between the resistant line DongNong40566 and the susceptible line DongNong410, self-crossing for three generations after F2. These SSR markers including Satt565、SOYGPATR and Satt396 were identified from500 SSR markers, the linkage order was Satt565—SOYGPATR—Hrcs1—Satt396, and the distance to the resistance locus was 12.7、6.5、14.7cM respectively. These three markers and the resistance locus were mapped to soybean linkage group C1.