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中国农学通报 ›› 2009, Vol. 25 ›› Issue (15): 0-1.

所属专题: 棉花

• 生物技术科学 •    下一篇

棉花黄萎病拮抗细菌2-70(Bacillus subtilis)菌株定殖能力的研究

李术娜,郭慧娟,王全,李红亚,王树香,王伟,朱宝成   

  • 收稿日期:2009-03-11 修回日期:2009-05-08 出版日期:2009-08-05 发布日期:2009-08-05

Studies on colonization of Antagonistic Bacteria 2-70(Bacillus subtilis)to Cotton Verticillium dahliae

  • Received:2009-03-11 Revised:2009-05-08 Online:2009-08-05 Published:2009-08-05

摘要:

本试验通过灭菌土和非灭菌土两种土样,试验前分别对这两种土样进行三种不同的处理即:用拮抗菌芽孢液浸种、土壤全部混菌和部分混菌进行盆栽试验。本文主要通过盆栽实验,考察棉花黄萎病拮抗细菌2-70在土壤中的定植情况及在棉花根内和根际的定植情况。结果表明,在实验室条件下,拮抗细菌2-70能够在灭菌土和非灭菌土中定殖,且定殖数量达106cfu/g土左右,经过五个月后仍能保持较高的抑菌活性,并且菌株在灭菌土中的定殖数量高于非灭菌土中的数量。通过对棉苗根内细菌的回收,证实拮抗细菌2-70能在棉花根内定殖,数量达到103cfu/g。

关键词: 甘蔗, 甘蔗, AFLP, 银染, PAGE

Abstract:

This test required sterile soil and non-sterile soil in two soil samples, test separately for the two soil samples of three different treatment that is: The antagonistic bacterium Bacillus soaking liquid, soil all mixed soil and some soil for potted plants mixed. In this paper, A pot experiment to study Verticillium dahliae 2-70 antagonistic bacteria in the soil and in the colonization of cotton roots and rhizosphere colonization of the situation. The results showed that under laboratory conditions, antagonistic bacteria can 2-70 in non-sterilized soil and sterilized soil colonization, and colonization Tatsu quantity 106cfu/g soil around, after five months has managed to maintain a higher antibacterial activity, and strains in sterile soil in the quantity colonization than non-sterilized soil in quantity. Cotton roots by bacteria recovery, confirmed the antagonistic bacteria in the 2-70 designated cotton root colonization, number of 103cfu/g.