欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (15): 0-.

所属专题： 生物技术； 水稻

• 生物技术科学 •    下一篇

水稻一个C3HC4型锌指蛋白编码基因的克隆表达与分析

刘丽华,林玲,鲁国东,王宗华,汪世华   

  • 收稿日期:2009-03-24 修回日期:2009-04-15 出版日期:2009-08-05 发布日期:2009-08-05

Cloning and characterization of OsZNP encoding a novel C3HC4-type zinc finger protein from rice

, , , ,   

  • Received:2009-03-24 Revised:2009-04-15 Online:2009-08-05 Published:2009-08-05

PDF (PC)

1086

摘要： 锌指蛋白是生物体内数量最多的转录调控因子，它在动植物的生长发育中都起着十分重要的作用。通过RT-RCR从水稻cDNA文库中获得一个水稻锌指蛋白基因OsZNP的完整阅读框，序列分析表明该基因编码一条253个氨基酸残基的多肽，含有一个典型的C3HC4型锌指结构。将测序正确的OsZNP基因克隆到表达载体pGEX-KG中，转化宿主菌BL21(DE3)，成功地构建了原核表达载体pGEX-KG-OsZNP。经IPTG诱导、SDS-PAGE电泳分析检测表明，水稻OsZNP基因以融合蛋白的形式在大肠杆菌中得到大量表达。

关键词: 生物制剂, 生物制剂, 氯嘧磺隆, 根系, 产量性状

Abstract: The zinc finger proteins belong to the largest family of regulatory transcription factors, which play an important role in growth and development in animal and plant systems. In this study, a novel zinc finger gene OsZNP was isolated from rice cDNA library by RT-PCR approach. Sequence analysis indicated that the gene encodes a 28 kDa protein with 253 amino acids and contains one typical C3HC4 zinc finger domains. The entire coding region of OsZNP gene was amplified by PCR. Then it was inserted into the prokaryotic fusion expression vector pGEX-KG to construct recombinant expression vector pGEX-KG-OsZNP, which was transformed into E. coil BL21. After the transformed bacteria were induced with IPTG, the expressed protein was analyzed by SDS-PAGE. The results showed that the target protein was expressed at a high level in E.coli BL21．