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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 17-20.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

羊驼AIF部分cDNA序列分析及其在不同毛色的表达定位

刘国敏,董常生   

  • 收稿日期:2009-05-15 修回日期:2009-06-04 出版日期:2009-08-20 发布日期:2009-08-20

The Sequence Analysis of Partial AIF Gene in Alpaca and the Gene Localization in Different Coat Color Trait

Liu Guomin, Dong Changsheng   

  • Received:2009-05-15 Revised:2009-06-04 Online:2009-08-20 Published:2009-08-20

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摘要： 通过对已构建的羊驼皮肤cDNA文库进行筛选和ESTs分析，发现与其它动物AIF基因相应cDNA序列高度同源的序列，经序列分析证明命名为羊驼AIF基因序列，已经向Genbank提交。与其他动物（褐鼠、小鼠、人等）的AIF基因相应序列进行相似性比较，相似性分别为100%、91%、71.6%。运用免疫组化技术进一步研究该基因在羊驼皮肤中的表达和定位，结果显示AIF在羊驼毛囊的毛根、根鞘、毛球、毛乳头位置均呈阳性表达，且棕色皮肤比灰色皮肤表达量高，提示该基因可能和羊驼毛色形成或毛的生长相关。

关键词: 红土晒烟, 红土晒烟, 移栽期, 产量, 品质

Abstract: A highly homologous sequence to AIF gene in Alpaca was obtained through selecting from established cDNA library of Alpaca skin and ESTs analysis. After verification by sequence analysis, the new gene was designated as AIF, and submitted to Genbank. In similarity comparison with AIFgene sequence of norway rat, mouse, and human being, the similarities were 100%, 91%, and 71.6%, respectively. Immunohistochemistry was applied for further investigating the expression and location of this gene in Alpaca skin. Results showed that positive expressions of AIF were found in hair root, root sheath, hair bulb, and dermal papilla, with higher expression in brown skin than grey skin. Our findings indicated that the gene could be related to the establishment of Alpaca coat color and hair growth.