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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (22): 0-0.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 •    

产蛋异常蛋鸭H9N2亚型禽流感病毒的分离鉴定

傅光华 程龙飞 施少华 陈红梅 林芳 林建生 黄瑜   

  • 收稿日期:2009-06-15 修回日期:2009-07-08 出版日期:2009-11-20 发布日期:2009-11-20
  • 基金资助:

    禽流感等重大动物疫病综合防控技术研究

Isolation and identification of H9N2 subtype avian influenza virus from egg-laying ducks with abnormal production

  • Received:2009-06-15 Revised:2009-07-08 Online:2009-11-20 Published:2009-11-20

PDF (PC)

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摘要：

从表现产蛋异常的蛋鸭中分离到1 株病毒, 经血凝抑制试验（HI）和聚合酶链反应（PCR）方法鉴定为H9N2亚型禽流感病毒。在GenBank中，BLAST分析扩增的该病毒3个基因片段表明，该株病毒与96年我国山东鸡H9N2亚型禽流感病毒(A/chicken/Shandong/6/96(H9N2))各相应的基因片段同源性最高，均为99%，所扩增的3个片段均为禽源H9N2亚型禽流感病毒的相应基因片段。对HA基因的遗传进化分析表明，该病毒与参考毒株（A/CK/BJ/94）处于同一进化分支上。

关键词: 葛藤, 葛藤, 根瘤菌, pH, 耐酸

Abstract:

A virus strain was isolated from samples of oviduct mucosa of breeder duck , which showed irregularity and reduction in egg-laying. The virus was identified as an H9N2 subtype avian influenza virus by haemagglutination inhibition test and polymerase chain reaction. Analysised by BLAST in GenBank database, the products of the PCR were all share 99% similarity with those of strain A/chicken/Shandong/6/96(H9N2), and the three genes fragment were all derived from the genes pool of H9N2 subtype avian influenza viruses. The phylogenetic analysis of HA gene showed that the fragment of the isolate was similar to that of the A/CK/BJ/94 lineage of H9N2 viruses.