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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (15): 0-.

所属专题： 食用菌； 食用菌

• 食品 营养 检测 安全 •    下一篇

藏灵菇酵母菌M3耐胃肠道逆环境特性及降胆固醇的实验研究

刘慧,陈湘宁,李晨,杨磊,张红星   

  • 收稿日期:2009-06-15 修回日期:2009-06-19 出版日期:2009-08-05 发布日期:2009-08-05

Study on Tolerance Characteristics of the Gastrointestinal Tract and Cholesterol-Lowering Effects of Kluyveromyces marxianus M3 from Tibetan Kefir

刘慧 LIU Hui, , , ,   

  • Received:2009-06-15 Revised:2009-06-19 Online:2009-08-05 Published:2009-08-05

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摘要： 摘 要：利用从藏灵菇中筛选的马克斯克鲁维酵母M3菌株探讨胃肠道耐受性及体外降胆固醇功效。分别在pH1.5~4.5、胆盐浓度0.3%~3.0%、NaCl浓度3.0%~6.0%的模拟胃肠道环境下，采用活菌计数法检测M3菌株的耐胃肠道逆环境特性，以邻苯二甲醛法检测其降胆固醇效果。结果表明：在pH1.5~4.5作用4h活菌数可达106cfu/ml，0.3%~3.0%胆盐及3.0%~6.0%NaCl作用8h活菌数可达105~106cfu/ml，在pH1.5~3.0(4h)及胆盐浓度0.3%~3.0%的模拟人体胃肠道环境下降胆固醇率均在60%以上。显示M3菌株具有较强的耐胃肠道逆环境特性及良好的降胆固醇能力，为开发研制降胆固醇功能食品及微生态制剂提供了实验基础。

关键词: 容重, 容重, 土壤孔隙度, 土类, 土壤板结, 化肥, 有机质

Abstract: Abstract: Using Kluyveromyces marxianus M3 isolated from Tibetan Kefir to study tolerance characteristics of the gastrointestinal tract and cholesterol-lowering effects in vitro. Viable count was used to detect M3 stains’ tolerance characteristics of the gastrointestinal tract separately under simulated gastrointestinal tract environment pH1.5~4.5, salt concentration 0.3~3.0%, sodium chloride 3.0~6.0%.At the same time, using O-phthalaldehyde to detect the effect of its cholesterol-lowering. The result shows that after 4 hours under pH1.5~4.5 the viable can get 106cfu/mlunder.The viable can be 105~106cfu/ml under 0.3%~3.0% bovine bile salt and 3.0%~6.0% sodium chloride(8h).Also, in the simulated gastrointestinal tract environment pH1.5~3.0(4h), salt concentration 0.3~3.0%(8h) the cholesterol-lowering can reach 60%. M3 strains have strong tolerance characteristics against the environment of the gastrointestinal tract and the good cholesterol-lowering ability, which provide the experimental basis for the development of cholesterol-lowering functional foods and probiotics’ study.