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中国农学通报 ›› 2009, Vol. 25 ›› Issue (22): 0-0.

所属专题: 生物技术 小麦

• 生物技术科学 •    

引进小麦种质材料的高分子量谷蛋白亚基分析

刘争辉 刘祎 何旭 杨起简 Novoselsksya-Dragovich A.Yu. Volodymyr S.Kravets   

  • 收稿日期:2009-06-19 修回日期:2009-07-07 出版日期:2009-11-20 发布日期:2009-11-20
  • 基金资助:
    国家科技支撑计划资助;科技部政府间合作项目;北京市国际合作项目

Analysis on the HMW-GS Components of introduced Wheat varieties

  • Received:2009-06-19 Revised:2009-07-07 Online:2009-11-20 Published:2009-11-20

摘要: 为了有效利用多年来引自俄罗斯及中亚地区的小麦资源,了解引进材料的遗传基础,特别是品质基础,采用SDS-PAGE技术对102份小麦材料的高分子量谷蛋白亚基(HMW-GS)组成进行了分析。结果表明,参试材料中共检测到11种HMW-GS类型,Glu-A1位点上有1、2*、Null,Null位点相对比较多,为40.20%;Glu-B1位点上有7、7+8、7+9、6+8、17+18,以7+9为主要类型(59.80%);Glu-D1位点有2+12、2+12’、5+10三种类型,其中5+10所占比例为51.96%。参试材料共检测到16种亚基组合,其中“Null,7+9,2+12”所占比例较大,为25.5%。值得一提的是,参试材料中品质评分为10分的材料有21个,9分的有29个。这些材料有可能会成为比较有价值的品质改良中间材料。

关键词: 甘蔗, 甘蔗, 能源作物, 燃料乙醇

Abstract: In order to make good use of foreign varieties, and to reallize the genetics base ,especially the quality traits of these introduced materials.HMW-GS compositions of 102 wheat cultivars from Russia and middle Asia area were analyzed by SDS-PAGE. The results showed that the number of allelic variations was eleven. At Glu-A1 locus, three alleles were detected, which encoded subunit 1, 2*and Null respectively and the major subunit was Null with the frequency of 40.20%;At Glu-B1 locus, five alleles were detected, which encoded7、7+8、7+9、6+8、17+18, the 7+9 was the major subunit pattern(59.80%). At Glu-D1 locus, there were three alleles which encoded 2+12 、2+12’ and 5+10 respectively were detected, and there are 51.96% materials took the subunit 5+10. Sixteen subunit combination patterns were identified and the major pattern was “Null, 7+9, 2+12”with the frequency of 25.5%.It is worth mentioning that there were twenty one cultivars with the score of 10 and twenty nine varieties with the score of 9 according to Payne’s scoring system.It was showed that these materials may be a promising resource for improving the bread-making quality of wheat in China.