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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (20): 58-62.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

苦马豆抗盐愈伤组织突变系的筛选

刘慧 杨军 王立强 陈国栋 沈顺   

  • 收稿日期:2009-06-26 修回日期:2009-07-21 出版日期:2009-10-20 发布日期:2009-10-20
  • 基金资助:

    四川省教育厅重点项目;四川省重点学科建设项目;四川省科技厅应用基础项目

Screening of Mutant Callus of Swainsona salsula Taubert Resistent to NaCl Stress

  • Received:2009-06-26 Revised:2009-07-21 Online:2009-10-20 Published:2009-10-20

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摘要：

本文以子叶外植体在MS培养基添加1.79 mg/L 2,4-D和1.15mg/L Kt诱导的愈伤组织为材料，添加不同浓度NaCl（100，200，280 mmol/L）下，经过不同时间（0，3，6，9天）胁迫处理，测定其可溶性蛋白含量、SOD和POD活性及丙二醛与脯氨酸含量，通过分析比较，筛选获得突变系的适宜盐浓度为200mmol/L NaCl。验证性试验表明， 200mmol/L NaCl胁迫下比100 mmol/L胁迫获得的抗性愈伤组织的数量多，而280 mmol/L胁迫下无抗性愈伤组织产生。本实验结果为获得抗盐再生植株及研究苦马豆的抗性机理奠定了基础。

关键词: 苜蓿, 苜蓿, 无菌苗, 下胚轴, 愈伤组织

Abstract:

Explants(cotyledons) was cultured on MS medium supplemented with 1.79 mg/L 2,4-D and 1.15 mg/L Kt for 36 days.And then, Induced callus were subcultured onto the medium with the same mediun plus NaCl in different concentration for salt stress, and the physiological characters (actvities of SOD and POD,contents of soluble protein, MDA and proline) of the callus were tested after0, 3, 6, 9 days of stress. By comparing physiological indexes from different stress, 200mmol/L NaCl was defined as the most suitable salt content for screening mutants resistent to salt stress. Experiments for verification showed that the amount of callus resistent to stress obtained from 200mmol/LNaCl was much more than that from 100mmol/LNaCl stress, while no resistent callus was achieved from 280 mmol/LNaCl stress, confirming the reliability of previous prediction. The results presented here provide the base for obtaining regenerated resistent plants and investigating the resistent mechanism of salt stress of this plant.