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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (20): 54-57.

所属专题： 生物技术； 小麦

• 生物技术科学 • 上一篇    下一篇

小麦春溶蛋白酸性聚丙烯酰胺凝胶电泳（A-PAGE）方法的优化与改良及其应用

王祖华 杨瑞先 姬云波   

  • 收稿日期:2009-05-25 修回日期:2009-06-15 出版日期:2009-10-20 发布日期:2009-10-20

Improvement and Its Application of Acid Polyacrylamide Gel Electrophoresis of Wheat Gliadin

  • Received:2009-05-25 Revised:2009-06-15 Online:2009-10-20 Published:2009-10-20

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摘要：

依据国际种子检验协会（ISTA）1986年公布的标准小麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳（A-PAGE）方法，结合国内实验室的实际情况，摸索出了适合我国试剂和仪器的小麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳（A-PAGE）的改良方法。该方法克服了标准方法中的胶韧性差、剥胶困难、分辨率不高、谱带识别繁琐、实验效率较低的限制，适合我国小麦品种纯度的快速鉴定、血缘关系分析等。

关键词: 郑州市郊区, 郑州市郊区, 城市化, 土地利用变化, 土壤功能, 演变

Abstract:

Based on the standard acid polyacrylamide gel electrophoresis of wheat gliadin was published by ISTA in 1986, combined with the actual circumstance of national laboratories, and explore the improved methods of the acid polyacrylamide gel electrophoresis of wheat gliadin of the reagents and equipment for our country. This method overcame the many restrictions of the standard method, such as the poor gel toughness, the gel desquamated uncomfortably, low resolution, troublesome identification of the bands, less efficiency of the experiment.,and suited the rapid identification and the consanguinity analysis of the purity of the Chinese wheat breed and so on.