欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (20): 63-67.

所属专题： 生物技术； 油料作物

• 生物技术科学 • 上一篇    下一篇

利用嫁接提高花生离体再生或转基因苗成活率的研究

李长生1,夏 晗1,卢金东2,李爱芹1,赵传志1,毕玉平1,王兴军1   

  • 收稿日期:2009-04-23 修回日期:2009-07-10 出版日期:2009-10-20 发布日期:2009-10-20
  • 基金资助:

    国家高技术研究发展计划项目;山东省农业科学院高技术自主创新基金;山东省农业科学院高技术自主创新基金

Graft Significantly Improves Survival Rate of Transgenic Peanut Plants

  • Received:2009-04-23 Revised:2009-07-10 Online:2009-10-20 Published:2009-10-20

PDF (PC)

1243

摘要：

花生（Arachis hypogaea L.）是世界上重要的油料作物。近年来，花生分子生物学发展迅速，但低效的再生和遗传转化系统严重制约着利用基因工程改良花生品种的研究。利用嫁接技术将组培苗或转基因苗嫁接到实生苗砧木上，不同嫁接方法成活率达到70-100%，远远高于转基因幼苗直接诱导生根移栽的成活率。

关键词: Cd2+, Cd2+, Pb2+, 木麻黄, 萌发, MDA, SOD

Abstract:

Peanut (Arachis hypogaea L.) is one of the most important oil crops in the world, belonging to the Leguminosae family and the Papilionacea subfamily, native to South America. Inefficiency of peanut regeneration system retarded its improvement through gene engineering. In this study we investigated the use of grafting to improve the survival rate of tissue cultured and transgenic plants. Plants of 14 days after germination were used as rootstock, and different grafting methods and plant management after grafting were investigated. The survival rate of different grafting methods ranged from 70-100% which is much higher than the survival rate obtained by directly transplanting transgenic plants after root induction.