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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (20): 68-71.

所属专题： 生物技术； 棉花

• 生物技术科学 • 上一篇    下一篇

农杆菌介导的植酸酶基因转化棉花的研究

郭彩菊 王省芬 张桂寅 迟吉娜 吴立强 李志坤 马峙英   

  • 收稿日期:2009-05-22 修回日期:2009-06-10 出版日期:2009-10-20 发布日期:2009-10-20

The Agrobacterium-Mediated PhyA Genetic Transformation for Embryogenic Calli of Cotton

  • Received:2009-05-22 Revised:2009-06-10 Online:2009-10-20 Published:2009-10-20

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摘要：

摘要：本研究采用农杆菌介导的方法，将外源植酸酶(Phy)基因导入棉花品种的胚性愈伤组织中，经培养获得再生植株。PCR检测证明植酸酶基因已经插入到棉花基因组中，晋棉14和陕724的转化效率分别为33%和8.3%。此外，本研究还对影响农杆菌侵染棉花胚性愈伤转化效率的因素，如卡那霉素浓度、胚性愈伤组织生长状态、受体材料的基因型等进行了较为系统的比较研究，旨在建立一种转化效率高、稳定性好的棉花农杆菌遗传转化体系。

关键词: 瓷玫瑰, 瓷玫瑰, 切花, 保鲜, 水分平衡

Abstract:

Abstract: In this study, some transgenic cotton plants with a phytase gene were obtained successfully via agrobacterium-mediated method of embryogenic calli. Transformation efficiency of resistant plants about Jimian14 and Shann724 was 33% and 8.3% respectively. Effect of kanamycin concentration, the growth status of embryogenic calli and the genotype of the target material on the transformation efficicency had been assayed. The factors influencing agrobacterium-mediated cotton calli transformation were studied systematically in order to establish a highly efficient and stable genetic transformation system of cotton.