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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (23): 308-312.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

药用植物红落藜组织培养研究

何碧珠 吴少华 邱万翔   

  • 收稿日期:2009-07-07 修回日期:2009-07-29 出版日期:2009-12-05 发布日期:2009-12-05

Studies on Plantlet Regeneration and Propagation of Chenopodium album

  • Received:2009-07-07 Revised:2009-07-29 Online:2009-12-05 Published:2009-12-05

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717

摘要： 本试验以红落藜为试验材料，进行茎段组织培养及植株再生的研究。试验结果表明75%酒精5s+0.1%升汞8min既可进行彻底消毒，又保证具有较高的成活率；通过初代培养筛选出NAA为最适的生长激素，最适培养基为MS + 6-BA 1.5 mg?L-1 + NAA 0.2mg?L-1+琼脂0.8%+蔗糖2%+活性炭0.2%；通过继代培养筛选出最适培养基为MS + 6-BA 2.0 mg?L-1 + NAA 0.2mg?L-1+琼脂0.8%+蔗糖2%+活性炭0.2%；最适的生根培养基为1/2MS + IBA1.0 mg?L-1 + NAA 0.1mg?L-1+琼脂0.8% + 蔗糖2% + 活性炭0.2%，生根率为80%。当试管苗叶片数3～5片，苗高2cm～3cm，生根数3～4条时，移栽至蛭石和腐殖土(1∶2)混合的基质中，保湿遮阴，成活率可达90％以上。

关键词: 砀山酥梨, 砀山酥梨, 树体改造, 果实品质

Abstract: The trial use the stems of Chenopodium L. for the tissue culture. With MS as the basic medium, after disinfection method, the proliferation of media, rooting medium screening, the test results shows that 75% alcohol 5 s +0.1% mercuric chloride 8 min to conduct a thorough disinfection, and ensure a higher survival rate ;MS+6-BA 1.5mg?L-1+ NAA 0.2mg?L-1+ agar 0.8% + sucrose 2% + activated charcoal 0.2% for primary culture, MS +6-BA 2.0mg?L-1+ NAA 0.2mg?L-1+ agar 0.8% + sucrose 2% + activated charcoal 0.2% for subculture, 1 / 2MS+IBA 1.0mg?L-1+ NAA 0.1mg?L-1+ agar 0.8% + sucrose 2% + activated charcoal 0.2% for the growing of roots, rooting rate of 80%.When the plantlets with 3 ~5 leaves, the height of 2~3 cm and 3 to 4 roots were transferred onto the mixture of Lennilite and Rotten breed soil(1∶2), and the survival rate was up to 90%.