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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (22): 0-0.

所属专题： 园艺

• 林学 园艺 园林 •    

平邑甜茶与扎矮山定子杂交后代的倍性鉴定及核型分析

周攀 张景娥 王颖 周晏起 王毅 周洪富 董文轩   

  • 收稿日期:2009-07-23 修回日期:2009-08-24 出版日期:2009-11-20 发布日期:2009-11-20
  • 基金资助:
    国家自然科学基金

Ploidy identification and karyotype analysis of the hybrids crossed by Pingyi Tiancha (Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang) and Zha’ai Shandingzi (Malus baccata (L.) Borkh.)

  • Received:2009-07-23 Revised:2009-08-24 Online:2009-11-20 Published:2009-11-20

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摘要： 以平邑甜茶与扎矮山定子杂交后代为试材，进行染色体观察和核型分析，以亲本和山定子为对照。结果表明：供试材料中6株皱叶型株系均为四倍体，2n=4x=68。核型公式分别为，2n= 4x= 44m + 24sm，2n= 4x= 60m + 8sm，2n= 4x= 20m + 48sm，2n= 4x= 52m + 16sm，2n= 4x= 56m + 12sm，2n= 4x= 48m + 20sm，核型分别为1B、2B、2A、2B、1A、1B类型。1株光叶株系为非整倍体，2n=43，核型公式为2n= 22m + 18sm + 3st，核型为2B类型。本研究为苹果无融合生殖型砧木育种提供了借鉴。

关键词: 小麦, 小麦, 品质, 近红外光谱技术

Abstract: Using the hybrids crossed by Pingyi Tiancha (Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang) and Zha’ai Shandingzi (Malus baccata (L.) Borkh.), the control plants of the two parents and normal M. baccata as the experimental materials to observe the chromosome numbers and analysis the karyotype. The results of the ploidy determination indicated that the ploidy of these six leaf-wrinkled plants are all tetraploidy: 2n=4x=68, and one smooth-leaf plant is aneupoid: 2n=43. The karyotype formulas are 2n=4x=44m+24sm, 2n=4x=60m+8sm, 2n=4x=20m+48sm, 2n=4x=52m+16sm, 2n=4x=56m+12sm, 2n=4x=48m+20sm and 2n=22m+18sm+3st, respectively. The karyotype types are 1B, 2B, 2A, 2B, 1A, 1B and 2B, respectively. Which will enrich the utilization and breeding of apomictic rootstock resources in Malus.