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中国农学通报 ›› 2009, Vol. 25 ›› Issue (23): 64-69.

• 生物技术科学 • 上一篇    下一篇

灵芝原生质体单核化育种

吴小平 刘 方 谢玉荣 王政龙 何桥兰 谢宝贵   

  • 收稿日期:2009-08-19 修回日期:2009-09-24 出版日期:2009-12-05 发布日期:2009-12-05

Protoplast Monokaryogenesis of Ganoderma lucidum Breeding

  • Received:2009-08-19 Revised:2009-09-24 Online:2009-12-05 Published:2009-12-05

摘要: 由于灵芝孢子难于在人工条件下萌发,无法获得灵芝育种所需的单核菌株,这使灵芝的育种进展缓慢。本实验利用生物统计软件和分子生物学方法,选择遗传距离较远和农艺性状互补的G.l0006 、G.l0012、G.l0024、G.l0030+2和G.l0003+1作为亲本菌株,通过原生质体单核化,获得了81株灵芝单核菌株,以此为材料进行杂交育,成功获得了24株杂交种,通过拮抗反应验证,证明它们确实是杂交菌株。

关键词: 小麦, 小麦, 品质, 三唑酮, 百菌清, 青霉素

Abstract: The spores of Ganoderma lucidum are difficult to germinate under the artificial conditions ,so it’s unable to get the monokaryon strains which is necessary in breeding of Ganoderma lucidum, and the advancement (progress )of breeding is slower. In this study, the statistical software and molecular biology methods were used to select the parents with genetic distance distant and complementary agronomic characters. In this way, the G.l0006, G.l0012, G.l0024, G.l0030+2, and G.l0003+1 were screened out as the hybrid parent strains. 81 mononuclear strains were obtained through protoplasted monokaryon technique, and they were used as hybrid breeding strains, 24 hybrids were obtained. The hybrids were verified to be different from their parents by antagonist response.